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Rapid determination of short chain carnitines in human plasma by electrospray ionisation-ion trap mass spectrometry using capillary electrophoresis instrument as sampler (Articolo in rivista)

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Rapid determination of short chain carnitines in human plasma by electrospray ionisation-ion trap mass spectrometry using capillary electrophoresis instrument as sampler (Articolo in rivista) (literal)

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Claudia Desiderio (a), Angelo Mancinelli (b), Antonella De Rossi (c), Diana Valeria Rossetti (d), Rosanna Inzitari (d), Irene Messana (e), Bruno Giardina (a,d), Massimo Castagnola (d) (2007)
Rapid determination of short chain carnitines in human plasma by electrospray ionisation-ion trap mass spectrometry using capillary electrophoresis instrument as sampler
in Journal of chromatography (Print)
(literal)

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Claudia Desiderio (a), Angelo Mancinelli (b), Antonella De Rossi (c), Diana Valeria Rossetti (d), Rosanna Inzitari (d), Irene Messana (e), Bruno Giardina (a,d), Massimo Castagnola (d) (literal)

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(a) Istituto di Chimica del Riconoscimento Molecolare-Sezione di Roma, Consiglio Nazionale delle Ricerche, c/o Istituto di Biochimica e Biochimica Clinica, Universita' Cattolica del Sacro Cuore, L.go F. Vito 1, 00168 Roma, Italy (b) Dipartimento Scientifico SIGMA-TAU, Via Pontina km 30,400, 00040 Pomezia, Roma, Italy (c) Istituto di Metodologie Chimiche, Consiglio Nazionale delle Ricerche, Area della Ricerca Roma 1, Via Salaria km 29.300,00016 Monterotondo Stazione, Roma, Italy (d) Istituto di Biochimica e Biochimica Clinica, Universit`a Cattolica del Sacro Cuore, L.go F. Vito 1, 00168 Roma, Italy (e) Dipartimento di Scienze Applicate ai Biosistemi, Universit`a di Cagliari, Campus Monserrato, 09042 Monserrato (Ca), Italy (literal)

Titolo

Rapid determination of short chain carnitines in human plasma by electrospray ionisation-ion trap mass spectrometry using capillary electrophoresis instrument as sampler (literal)

Abstract

A capillary electrophoresis apparatus was used as sampler for flow injection analysis (FIA) in tandem mass spectrometry of l-carnitine and its acetyl- and propionyl-metabolites in human plasma. The capillary electrophoresis instrument was coupled to the ion trap mass spectrometer by an electrospray ionization coaxial sheath liquid interface. The electrophoresis capillary introduced the sample directly into the source by applying a prolonged sample injection. The use of the capillary electrophoresis apparatus miniaturised the FIA procedure, substantially reducing the quantities of solvents and samples used, and allowed rapid automated sequential analyses. The method was optimised and validated using a dialyzed human plasma matrix. The plasma samples were analysed after a simple, rapid deproteinisation procedure with acetonitrile and diluted 70 times before direct injection into the mass spectrometer for product ion scan MS/MS analysis in positive ionisation. The total analysis time was 5 min, including capillary preconditioning and acquisition time (3 min). The method was sensitive, allowing the determination of l-, l-acetyl- and l-propionyl-carnitines at 140, 14 and 3.6nM concentrations (injected values) corresponding to lower limit of quantitation values in plasma of 10, 1 and 0.25?M, respectively. The method was processed for full validation and applied to the analysis of l-carnitine and its short chain derivatives in human plasma samples. (literal)

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