Replacement of isobutyl by trifluoromethyl in pepstatin A selectively affects inhibition of aspartic proteinases (Articolo in rivista)

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  • Replacement of isobutyl by trifluoromethyl in pepstatin A selectively affects inhibition of aspartic proteinases (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/cbic.200500180 (literal)
Alternative label
  • Binkert C., Frigerio M., Jones A., Meyer S., Pesenti C., Prade L., Viani F., Zanda M. (2006)
    Replacement of isobutyl by trifluoromethyl in pepstatin A selectively affects inhibition of aspartic proteinases
    in ChemBioChem (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Binkert C., Frigerio M., Jones A., Meyer S., Pesenti C., Prade L., Viani F., Zanda M. (literal)
Pagina inizio
  • 181 (literal)
Pagina fine
  • 186 (literal)
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  • http://biblioproxy.cnr.it:2052/doi/10.1002/cbic.200500180/abstract (literal)
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  • 7 (literal)
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  • 6 (literal)
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  • 1 (literal)
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  • Scopus (literal)
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  • Christoph Binkert Dr.1, Massimo Frigerio2, Andrew Jones1, Solange Meyer Dr.1, Cristina Pesenti Dr.2, Lars Prade Dr.1, Fiorenza Viani Dr.2, Matteo Zanda Dr.21Actelion Pharmaceuticals Ltd. Gewerbestrasse 16, 4123 Allschwil, Switzerland, Fax: (+41) 61-487-4500 2C.N.R.--Istituto di Chimica del Riconoscimento Molecolare (literal)
Titolo
  • Replacement of isobutyl by trifluoromethyl in pepstatin A selectively affects inhibition of aspartic proteinases (literal)
Abstract
  • Two bis-trifluoromethyl pepstatin A analogues, carboxylic acid 1 and its methyl ester 2, have been synthesised in order to probe the properties and size of the trifluoromethyl (Tfm) group and compare it to the \"bigger\" isobutyl that is present in pepstatin A. The results demonstrate that Tfm can effectively replace the isobutyl chain as far as inhibitory activity against plasmepsin II (PM II), an aspartic proteinase from Plasmodium falciparum, is concerned. On the other hand, replacement of isobutyl by Tfm selectively affected activity against other aspartic proteinases tested. Two lines of evidence led to these conclusions. Firstly, compounds 1 and 2 retained single-digit nanomolar inhibitory activity against PM II, but were markedly less active against PM IV, cathepsin D and cathepsin E. Secondly, the X-ray crystal structures of the three complexes of PM II with 1, 2 and pepstatin A were obtained at 2.8, 2.4 and 1.7 Å resolution, respectively. High overall similarity among the three complexes indicated that the central Tfm was well accommodated in the lipophilic S1 pocket of PM II, where it was involved in tight hydrophobic contacts. The interaction of PM II with Phe111 appeared to be crucial. Comparison of the crystal structures presented here, with X-ray structures or structural models of PM IV and cathepsin D, allowed an interpretation of the inhibition profiles of pepstatin A and its Tfm variants against these three enzymes. Interactions of the P1 side chain with amino acids that point into the S1 pocket appear to be critical for inhibitory activity. In summary, Tfm can be used to replace an isobutyl group and can affect the selectivity profile of a compound. These findings have implications for the design of novel bioactive molecules and synthetic mimics of natural compounds. (literal)
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