Analysis of interactions of protease inhibitors with proteases in a high-throughput format. (Abstract/Poster in atti di convegno)

Type
Label
  • Analysis of interactions of protease inhibitors with proteases in a high-throughput format. (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2003-01-01T00:00:00+01:00 (literal)
Alternative label
  • Greco S; Volpicella M; Ceci LR; Logrieco A; Kludkiewicz B; Poltronieri P; Santino A (2003)
    Analysis of interactions of protease inhibitors with proteases in a high-throughput format.
    in BIOARRAYS EUROPE 2003, Cambridge, 2-4.10.2003
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Greco S; Volpicella M; Ceci LR; Logrieco A; Kludkiewicz B; Poltronieri P; Santino A (literal)
Note
  • Poster (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Institute of Crop Protection, Ceske Budejovice, Czech Republic Università di Bari e del Salento Polish Academy of Science, Inst. Biochemistry Biophysics, Warsawa (literal)
Titolo
  • Analysis of interactions of protease inhibitors with proteases in a high-throughput format. (literal)
Abstract
  • A collection of protease inhibitors was screened forthe interaction with various serine (trypsin, cathepsin B) and cysteine (papain, ficin, bromelain) proteases possessing cleavage activity towards a Dabcyl pentapeptide fluorogenic substrate. Protease inhibitor's instability (constant of protease-inhibitor complex) was determined prior to their use in the arrayed format. The inhibitors used were: St-CYST, A. thaliana cysteien protease inhibitor); MTI2, mustard trypsin inhibitor; MTI-2-AAA mutant without inhibitory activity; MTI-2-PLI mustard chymotrypsin inhibitor; MTI-2-PAI trypsin inhibitor; Chymo8, chymotrypsin inhibitor; RTI-2, rapeseed glutamyl protease inhibitor;Silk protease inhibitors (six variants with one modified amino acid in the P1 site: thr, Trp, Ala, His, Met, Asp, with various efficiencies in inhibiting elastase, proteinase K, thermolysin and tryspin; Sb-RI, soybean trypsin inhibitor. Inhibitors were spotted onto Schleicher-Schuell nitrocelulose slides, and proteases were labelled with cyanine-3 before hybridisation with slides. Data presented show the specificity of binding to each inhibitor and on activity of proteases. (literal)
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