Cloning maff by Recognition Site Screening with the NFE2 Tandem Repeat of HS2: Analysis of Its Role in Globin and gcsl Genes Regulation (Articolo in rivista)

Type
Label
  • Cloning maff by Recognition Site Screening with the NFE2 Tandem Repeat of HS2: Analysis of Its Role in Globin and gcsl Genes Regulation (Articolo in rivista) (literal)
Anno
  • 2002-01-01T00:00:00+01:00 (literal)
Alternative label
  • Marini M. G., Asunis I., Chan K., Chan J. Y., Kan Y.W., Porcu L., Cao A., Moi P. (2002)
    Cloning maff by Recognition Site Screening with the NFE2 Tandem Repeat of HS2: Analysis of Its Role in Globin and gcsl Genes Regulation
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Marini M. G., Asunis I., Chan K., Chan J. Y., Kan Y.W., Porcu L., Cao A., Moi P. (literal)
Pagina inizio
  • 145 (literal)
Pagina fine
  • 158 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 29 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Il lavoro descrive l'isolamento del fattore di trascrizione MafF e la sua caratterizzazione funzionale. I dati dimostrano che MafF si associa con altri fattori di trascrizione appartenenti alla famiglia CNC e regola i geni globinici in misura modesta. Alcuni geni della fase II della risposta agli stress ossidativi sono invece attivati in misura più evidente, soprattutto a seguito della eterodimerizzazione col fattore di trascrizione c-jun. La regolazione dei geni della fase II implica un coinvolgimento di questo fattore nei processi di detossificazione dei farmaci, di prodotti xenobiotici e in ultima analisi nella prevenzione dei tumori. (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituto di Neurogentica e Neurofarmacologia - CNR Cagliara Dipartimento di Scienze Biomediche e Biotecnologie . Università di Cagliari Howard Hughes Medical Institute - UCSF - San francisco (literal)
Titolo
  • Cloning maff by Recognition Site Screening with the NFE2 Tandem Repeat of HS2: Analysis of Its Role in Globin and gcsl Genes Regulation (literal)
Abstract
  • The erythroid-specific enhancer within hypersensitivity site 2 (HS2) of the human beta-globin locus control region is required for high level globin gene expression. We used an oligonucleotide of the NF-E2 tandem repeat, within HS2, as recognition site probe to screen a K562 cDNA library for interacting transcription factors. A 2.3 kb full length cDNA encoding the b-zip transcription factor MafF was isolated. MafF can form both homodimers and high affinity heterodimers with Nrf1, Nrf2 and Nf-E2, three members of the CNC-bZip family. Despite obvious structural similarities with the other small Maf proteins, MafF differs in its tissue distribution and its inability to repress transcription when overexpressed as homodimer. In fact, in different cell lines and on different promoters (beta-globin, gamma-globin and glutamylcysteine synthetase genes) the MafF homodimers do not appreciably affect transcription of target promoters, whereas MafF/CNC member heterodimers act as weak transcriptional activators. Even though MafF was cloned using probes derived from the globin LCR, it is in the context of the GCSl promoter and in combination with Jun that MafF shows a rather distinct and specific regulatory role. These observations suggest that a complex network of small Maf and CNC?AP1 protein interactions might be involved in regulating transcription in diverse tissues or developmental stages. (literal)
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