A DIGE approach for the assessment of rat soleus muscle changes during unloading: effect of acetyl-L-carnitine supplementation (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• A DIGE approach for the assessment of rat soleus muscle changes during unloading: effect of acetyl-L-carnitine supplementation (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/pmic.200701176 (literal)

Alternative label

• Moriggi M (1); Cassano P (2); Vasso M (1,3); Capitanio D (1); Fania C (1,3); Musicco C (4); Pesce V (2); Gadaleta MN (2,4); Gelfi C (1,3). (2008)
  A DIGE approach for the assessment of rat soleus muscle changes during unloading: effect of acetyl-L-carnitine supplementation
  in Proteomics (Weinh., Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Moriggi M (1); Cassano P (2); Vasso M (1,3); Capitanio D (1); Fania C (1,3); Musicco C (4); Pesce V (2); Gadaleta MN (2,4); Gelfi C (1,3). (literal)

Pagina inizio

• 3588 (literal)

Pagina fine

• 3604 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• Subject Category: Biochemistry & Molecular Biology (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 8 (literal)

Rivista

• Proteomics (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• Pubblicato on-line: 6 Agosto 2008 Corresponding author: C. Gelfi (cecilia.gelfi@unimi.it) I.F.: 4.586 (literal)

Note

• Google Scholar (literal)
• PubMe (literal)
• ISI Web of Science (WOS) (literal)
• Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1. Department of Sciences and Biomedical Technologies, University of Milan, Segrate (MI), Italy 2. Department of Biochemistry and Molecular Biology Ernesto Quagliariello, University of Bari, Bari, Italy 3. Institute of Molecular Bioimaging and Physiology, National Research Council, Segrate (MI), Italy 4. Institute of Biomembranes and Bioenergetics, CNR, Bari, Italy (literal)

Titolo

• A DIGE approach for the assessment of rat soleus muscle changes during unloading: effect of acetyl-L-carnitine supplementation (literal)

Abstract

• After hind limb suspension, a remodeling of postural muscle phenotype is observed. This remodeling results in a shift of muscle profile from slow-oxidative to fast-glycolytic. These metabolic changes and fiber type shift increase muscle fatigability. Acetyl-L-carnitine (ALCAR) influences the skeletal muscle phenotype of soleus muscle suggesting a positive role of dietary supplementation of ALCAR during unloading. In the present study, we applied a 2-D DIGE, mass spectrometry and biochemical assays, to assess qualitative and quantitative differences in the proteome of rat slow-twitch soleus muscle subjected to disuse. Meanwhile, the effects of ALCAR administration on muscle proteomic profile in both unloading and normal-loading conditions were evaluated. The results indicate a modulation of troponin I and tropomyosin complex to regulate fiber type transition. Associated, or induced, metabolic changes with an increment of glycolytic enzymes and a decreased capacity of fat oxidation are observed. These metabolic changes appear to be counteracted by ALCAR treatment, which restores the mitochondrial mass and decreases the glycolytic enzyme expression, suggesting a normalization of the metabolic shift observed in unloaded animals. This normalization is accompanied by a maintenance of body weight and seems to prevent a switch of fiber type. (literal)

Prodotto di

• Genomica e proteomica per la ricerca di biomarcatori in malattie neurodegenerative e nei tumori (ME.P05.002.001) (Modulo)
• Biogenesi delle Membrane di Trasduzione dell'Energia. (SV.P13.002.001) (Modulo)
• Istituto di biomembrane e bioenergetica (IBBE) (Istituto)
• Institute of molecular bioimaging and physiology (IBFM) (Istituto)

Autore CNR

• MICHELE VASSO (Persona)
• MARIA NICOLA GADALETA (Persona)
• CHIARA FANIA (Persona)
• DANIELE CAPITANIO (Unità di personale esterno)
• CECILIA GELFI (Unità di personale esterno)
• CLARA MUSICCO (Persona)

Insieme di parole chiave

• Keywords of "A DIGE approach for the assessment of rat soleus muscle changes during unloading: effect of acetyl-L-carnitine supplementation" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di biomembrane e bioenergetica (IBBE) (Istituto)
• Institute of molecular bioimaging and physiology (IBFM) (Istituto)
• Biogenesi delle Membrane di Trasduzione dell'Energia. (SV.P13.002.001) (Modulo)
• Genomica e proteomica per la ricerca di biomarcatori in malattie neurodegenerative e nei tumori (ME.P05.002.001) (Modulo)

Autore CNR di

• CLARA MUSICCO (Persona)
• MARIA NICOLA GADALETA (Persona)
• MICHELE VASSO (Persona)
• CHIARA FANIA (Persona)
• DANIELE CAPITANIO (Unità di personale esterno)
• CECILIA GELFI (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Proteomics (Rivista)

Insieme di parole chiave di

• Keywords of "A DIGE approach for the assessment of rat soleus muscle changes during unloading: effect of acetyl-L-carnitine supplementation" (Insieme di parole chiave)