http://www.cnr.it/ontology/cnr/individuo/prodotto/ID170963
Electrochemical impedance spectroscopy and fluorescence lifetime imaging of lipid mixtures self-assembled on mercury (Articolo in rivista)
- Type
- Label
- Electrochemical impedance spectroscopy and fluorescence lifetime imaging of lipid mixtures self-assembled on mercury (Articolo in rivista) (literal)
- Anno
- 2010-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1039/b923895f (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Lucia Becucci; Stefano Martinuzzi; Emanuela Monetti; Raffaella Mercatelli; Franco Quercioli;
Dario Battistel; and Rolando Guidelli (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
- http://pubs.rsc.org/en/content/articlelanding/2010/sm/b923895f (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
- fasc.(12). Royal Society of Chemistry. (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Department of Chemistry, Florence University, Via della Lastruccia 3,
50019 Sesto Fiorentino, Firenze
ISC, Istituto dei Sistemi Complessi del CNR, CNR, Via Madonna del
Piano, 10, 50019 Sesto Fiorentino, Firenze
Department of Physical Chemistry, Venice University, Calle Larga
S. Marta 2137, 30123 Venezia (literal)
- Titolo
- Electrochemical impedance spectroscopy and fluorescence lifetime imaging of lipid mixtures self-assembled on mercury (literal)
- Abstract
- Monolayers and bilayers of lipid mixtures self-assembled on mercury form spontaneously gel-phase (solid ordered, s(o)) and liquid-ordered (l(o)) microdomains, thanks to the fluidity imparted to these films by the liquid metal support. The differential capacity of the hydrocarbon tail region of monolayers of mixtures of two lipid components of high and low transition temperature T-m, increases during the transition from the liquid disordered (l(d)) phase to the coexistence of l(d) and s(o) phases. Addition of cholesterol to this binary mixture causes a decrease in differential capacity. This behavior is explained by regarding the capacity as a measure of the total perimeter of the s(o) microdomains, due to the mismatch between these microdomains and the l(d) phase. Cholesterol removes this mismatch by converting the anisotropic s(o) microdomains into isotropic l(o) microdomains (rafts). This allows differential capacity measurements by electrochemical impedance spectroscopy to follow phase transitions in lipid mixtures. The coexistance of l(d), l(o) and s(o) phases is confirmed by images of a distal lipid monolayer self-assembled on top of a thiolipid monolayer tethered to a mercury microcap, by using two-photon fluorescence lifetime imaging microscopy (2P-FLIM). (literal)
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