Characterization of BSA unfolding and aggregation using a Single-Capillary Viscometer and Dynamic Surface Tension Detector (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Characterization of BSA unfolding and aggregation using a Single-Capillary Viscometer and Dynamic Surface Tension Detector (Articolo in rivista) (literal)

Anno

• 2011-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.talanta.2011.08.009 (literal)

Alternative label

• Bramanti Emilia [ 1 ]; Ferrari Carlo [ 2 ]; Angeli Valeria [ 1 ], Onor Massimo [ 1]; Synovec Robrt E. [ 3 ] (2011)
  Characterization of BSA unfolding and aggregation using a Single-Capillary Viscometer and Dynamic Surface Tension Detector
  in Talanta (Oxf.)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Bramanti Emilia [ 1 ]; Ferrari Carlo [ 2 ]; Angeli Valeria [ 1 ], Onor Massimo [ 1]; Synovec Robrt E. [ 3 ] (literal)

Pagina inizio

• 2553 (literal)

Pagina fine

• 2561 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 85 (literal)

Rivista

• Talanta (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 5 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• [ 1 ] Ist Chim Composti Organo Metall ICCOM UOS Pisa, CNR, Natl Res Council Italy, Area Ric, I-56124 Pisa, Italy [ 2 ] INO UOS Pisa, Ist Nazl Ott, CNR, Natl Res Council Italy,Area Ric, I-56124 Pisa, Italy [ 3 ] Univ Washington, Ctr Proc Analyt Chem, Dept Chem, Seattle, WA 98195 USA (literal)

Titolo

• Characterization of BSA unfolding and aggregation using a Single-Capillary Viscometer and Dynamic Surface Tension Detector (literal)

Abstract

• A dynamic surface tension detector (DSTD) has been equipped with an additional pressure sensor for simultaneous viscosity measurements, as a detector for flow injection analysis. The viscosity measurement is based on a single capillary viscometer (SCV) placed in parallel configuration with the DSTD. The viscometer in the optimized conditions consists of a PEEK capillary (i.d.=0.25 mm, L=75 cm) kept at constant temperature using a thermostatic bath, which leads on the two sides to the two arms of a differential piezoelectric pressure transducer with a range of 0-35 psi. The DSTD, described previously, measures the changing pressure across the liquid/air interface of 2 mu L. drops repeatedly forming at the end of a capillary. SCV performance has been evaluated by measuring dynamic viscosity of water/glycerol mixtures analysed in flow injection and comparing the results with the values reported in the literature. The detection limits of SCV and DSTD, calculated as 3 sigma of the blank, were 0.012 cP and 0.6 dyn cm(-1), respectively. The Fl-SCV-DSTD system has been applied to the study of temperature-induced denaturation/aggregation process in bovine serum albumin (BSA). The results have been supported and discussed with respect to BSA conformational analysis performed using Fourier Transform infrared spectroscopy. (literal)

Prodotto di

• Istituto di chimica dei composti organo metallici (ICCOM) (Istituto)
• Interazione coerente di radiazione con atomi, molecole e superfici attraverso la progettazione e sviluppo di nuove metodologie e sorgenti dall'UV al millimetrico (MD.P03.014.002) (Modulo)
• Chimica analitica strumentale (PM.P03.015.002) (Modulo)

Autore CNR

• MASSIMO ONOR (Persona)
• Valeria Angeli (Persona)
• EMILIA BRAMANTI (Persona)
• CARLO FERRARI (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Characterization of BSA unfolding and aggregation using a Single-Capillary Viscometer and Dynamic Surface Tension Detector" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di chimica dei composti organo metallici (ICCOM) (Istituto)
• Interazione coerente di radiazione con atomi, molecole e superfici attraverso la progettazione e sviluppo di nuove metodologie e sorgenti dall'UV al millimetrico (MD.P03.014.002) (Modulo)
• Chimica analitica strumentale (PM.P03.015.002) (Modulo)

Autore CNR di

• MASSIMO ONOR (Persona)
• EMILIA BRAMANTI (Persona)
• CARLO FERRARI (Unità di personale interno)
• Valeria Angeli (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Talanta (Rivista)

Insieme di parole chiave di

• Parole chiave di "Characterization of BSA unfolding and aggregation using a Single-Capillary Viscometer and Dynamic Surface Tension Detector" (Insieme di parole chiave)