Antibodies conjugated with new highly luminescent Eu3+ and Tb3+ chelates as markers for time resolved immunoassays. Application to simultaneous determination of clenbuterol and free cortisol in horse urine (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Antibodies conjugated with new highly luminescent Eu3+ and Tb3+ chelates as markers for time resolved immunoassays. Application to simultaneous determination of clenbuterol and free cortisol in horse urine (Articolo in rivista) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Alternative label

• Bacigalupo, M.A.; Meroni, G.; Secundo, F.; Scalera, C.; Quici, S. (2009)
  Antibodies conjugated with new highly luminescent Eu3+ and Tb3+ chelates as markers for time resolved immunoassays. Application to simultaneous determination of clenbuterol and free cortisol in horse urine
  in Talanta (Oxf.)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Bacigalupo, M.A.; Meroni, G.; Secundo, F.; Scalera, C.; Quici, S. (literal)

Pagina inizio

• 954 (literal)

Pagina fine

• 958 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 80 (literal)

Rivista

• Talanta (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• DOI: 10.1016/j.talanta.2009.08.019 (literal)

Note

• ISI Web of Science (WOS) (literal)

Titolo

• Antibodies conjugated with new highly luminescent Eu3+ and Tb3+ chelates as markers for time resolved immunoassays. Application to simultaneous determination of clenbuterol and free cortisol in horse urine (literal)

Abstract

• Highly luminescent Eu3+ and Tb3+ complexes of 10-[4-(3-isothiocyanatopropoxy)benzoylmethyl]-1,4,7,10-tetraazacyclodode cane-1,4,7 triacetic acid Eu3+ subset of 1 and Tb3+ subset of 1 were conjugated with a goat anti-rabbit IgG and a rabbit anti-mouse IgG, respectively, and applied as markers in a time resolved immunoassay for simultaneous quantitative determination of anabolic compounds clenbuterol (CL) and hydrocortisone (HC). The assay was performed in horse urine, using a monoclonal antibody specific to CL and a rabbit polyclonal antibody specific to the free HC. These lanthanide chelates are very stable and highly luminescent in aqueous solution and allowed to reach 10 mu g L-1 and 40 mu g L-1 sensitivities for CL and for HC, respectively. Application to the horse urine, that is a very complex matrix, has a considerable interest in the control of illegal use of these compounds. (literal)

Prodotto di

• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)
• Applicazioni sintetiche della biocatalisi (PM.P03.009.002) (Modulo)
• Componenti molecolari, supramolecolari o macromolecolari con proprietà fotoniche ed optoelettroniche (PM.P04.004.001) (Modulo)
• Istituto di scienze e tecnologie molecolari (ISTM) (Istituto)

Autore CNR

• CHIARA SCALERA (Unità di personale esterno)
• SILVIO QUICI (Unità di personale interno)
• MARIA ANGELA BACIGALUPO (Persona)
• FRANCESCO SECUNDO (Persona)
• GIACOMO MERONI (Persona)

Incoming links:

Prodotto

• Istituto di scienze e tecnologie molecolari (ISTM) (Istituto)
• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)
• Applicazioni sintetiche della biocatalisi (PM.P03.009.002) (Modulo)
• Componenti molecolari, supramolecolari o macromolecolari con proprietà fotoniche ed optoelettroniche (PM.P04.004.001) (Modulo)

Autore CNR di

• SILVIO QUICI (Unità di personale interno)
• GIACOMO MERONI (Persona)
• MARIA ANGELA BACIGALUPO (Persona)
• FRANCESCO SECUNDO (Persona)
• CHIARA SCALERA (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Talanta (Rivista)