A dimerizable cationic lipid with potential for gene delivery (Articolo in rivista)

Type
Label
  • A dimerizable cationic lipid with potential for gene delivery (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Alternative label
  • Candiani G., Pezzoli D., Cabras M., Ristori S., Pellegrini C., Kajaste-Rudnitski A., Vicenzi E., Sala C., Zanda M. (2008)
    A dimerizable cationic lipid with potential for gene delivery
    in The journal of gene medicine
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Candiani G., Pezzoli D., Cabras M., Ristori S., Pellegrini C., Kajaste-Rudnitski A., Vicenzi E., Sala C., Zanda M. (literal)
Pagina inizio
  • 637 (literal)
Pagina fine
  • 645 (literal)
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  • 10 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Gabriele Candiani1,2* Daniele Pezzoli1 Mariasara Cabras1 Sandra Ristori3 Cinzia Pellegrini1 Anna Kajaste-Rudnitski4 Elisa Vicenzi4 Carlo Sala5 Matteo Zanda1** 1CNR, Istituto di Chimica del Riconoscimento Molecolare, Milan, Italy 2BioCell, Department of Chemistry, Materials and Chemical Engineering 'G. Natta', Politecnico di Milano, Milan, Italy 3Department of Chemistry, University of Florence, Sesto Fiorentino, Italy 4Viral Pathogens and Biosafety Unit, San Raffaele Scientific Institute, Milan, Italy 5CNR, Institute of Neuroscience, Cellular and Molecular Pharmacology, Milan, Italy (literal)
Titolo
  • A dimerizable cationic lipid with potential for gene delivery (literal)
Abstract
  • Background Despite the use of currently optimized lipofection conditions, including transfection in serum-depleted media, the efficiency of gene transfer is low and high transfection rates often induce cytotoxicity. A lipid formulation with transfection efficiency not inhibited by serum would provide an advance towards in vivo applications. Methods We explored the ability of the cationic lipid SH-14 to dimerize upon DNA and form lipoplexes, and potentially release nucleic acids in the intracellular reducing milieu. We investigated the critical micelle-forming concentration of SH-14 and its intrinsic toxicity, size and Zeta potential measurements, the in vitro cytotoxicity of SH-14/plasmid DNA lipoplexes and their ability to transfect cells. Results Among all the charge ratios (CR, +/-) tested, lipoplexes at CR 10 with a mean diameter of 295 nm and a surface charge of +20 mV, exhibited the best compromise between transfection efficiency and tolerability. SH-14 presented the same cytotoxicity level whether alone or complexed in lipoplexes. Lipofections carried out in serum-free medium shared a transfection. efficiency, on average, of 40% and a cytotoxicity of 38%. An increase of 73% in transfection efficiency and 24% in cell viability were obtained, extending lipofection over 48 h in complete-medium. Moreover, when serum concentration was increased from 10% to 50%, a three-fold increase in plasmid dose led to more than 72% of cells being transfected with almost no sign of cytotoxicity. (literal)
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