Apyap1 affects aflatoxin biosynthesis during Aspergillus parasiticus growth in maize seeds (Articolo in rivista)

Type
Label
  • Apyap1 affects aflatoxin biosynthesis during Aspergillus parasiticus growth in maize seeds (Articolo in rivista) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1080/02652030701553244 (literal)
Alternative label
  • Reverberi M., Zjalic S., Punelli F., Ricelli A., Fabbri A.A., Fanelli C. (2007)
    Apyap1 affects aflatoxin biosynthesis during Aspergillus parasiticus growth in maize seeds
    in Food additives and contaminants
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Reverberi M., Zjalic S., Punelli F., Ricelli A., Fabbri A.A., Fanelli C. (literal)
Pagina inizio
  • 1070 (literal)
Pagina fine
  • 1075 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 24 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Reverberi M., Zjalic S., Punelli F., Fabbri A. A., Fanelli C. - Department of Plant Biology, University “Università degli Studi \"La Sapienza”, Largo Cristina di Svezia, 24 00165 Rome (Italy); Ricelli A. - CNR, Institute of Science of Food Production, via G. Amendola, 122/O, 70126 Bari (Italy). (literal)
Titolo
  • Apyap1 affects aflatoxin biosynthesis during Aspergillus parasiticus growth in maize seeds (literal)
Abstract
  • It is demonstrated that, in fungal cells grown in synthetic media, the Apyap1 gene is implicated in the modulation of aflatoxin biosynthesis following the perturbation of redox balance. This study suggests that an association between oxidative stress and aflatoxin biosynthesis also occurs in maize seeds. We used ”Apyap1, a strain in which the gene Apyap1 was disrupted, to verify whether this oxidative stress-related transcription factor, by affecting cell redox balance, can have a role in the modulation of aflatoxin synthesis. The amount of hydroperoxides (ROOH) produced by wild type (WT) and ”Apyap1, both grown in potato dextrose broth, was assayed in the filtrate. In maize seeds (30 g), inoculated with WT and ”Apyap1conidia and incubated at 30°C for 15 days, lipoxygenase activity (LOX), lipoperoxides (LOOH) production, fungal growth and aflatoxin biosynthesis was analysed. It was observed that ”Apyap1 released more hydroperoxides in the culture media and more aflatoxins in seeds, possibly through stronger stimulation of LOX, which, in turn led to greater LOOH production in the seeds. On the basis of the results, a hypothesis regarding strategies to control aflatoxin ........... (literal)
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