http://www.cnr.it/ontology/cnr/individuo/prodotto/ID169873
Towards the analysis of the genomes of single cells: further characterisation of the multiple displacement amplification (Articolo in rivista)
- Type
- Label
- Towards the analysis of the genomes of single cells: further characterisation of the multiple displacement amplification (Articolo in rivista) (literal)
- Anno
- 2006-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.gene.2006.01.032 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Simona Panelli; Giuseppe Damiani; Luca Espen; Gioacchino Micheli; Vittorio Sgaramella (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
- http://www.sciencedirect.com/science/article/pii/S0378111906001430 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
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- PubMe (literal)
- Google Scholar (literal)
- Scopu (literal)
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- * CERSA/Fondazione Parco Tecnologico Padano, via Einstein 2, Lodi, Italy.
* IGM-CNR, via Ferrata 1, Pavia, Italy.
* Dipartimento di Produzione Vegetale, Università di Milano, Italy.
* IBPM-CNR, Piazzale A. Moro, 5, Roma, Italy.
* CERSA/Fondazione Parco Tecnologico Padano, via Einstein 2, Lodi, Italy. (literal)
- Titolo
- Towards the analysis of the genomes of single cells: further characterisation of the multiple displacement amplification (literal)
- Abstract
- The development of methods for the analysis and comparison of the nucleic acids contained in single cells is an ambitious and challenging goal that may provide useful insights in many physiopathological processes. We review here some of the published protocols for the amplification of whole genomes (WGA). We focus on the reaction known as Multiple Displacement Amplification (MDA), which probably represents the most reliable and efficient WGA protocol developed to date. We discuss some recent advances and applications, as well as some modifications to the reaction, which should improve its use and enlarge its range of applicability possibly to degraded genomes, and also to RNA via complementary DNA. (literal)
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