Speciation analysis of selenoproteins in human serum by solid-phase extraction and affinity HPLC hyphenated to ICP-quadrupole MS (Articolo in rivista)

Type
Label
  • Speciation analysis of selenoproteins in human serum by solid-phase extraction and affinity HPLC hyphenated to ICP-quadrupole MS (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Alternative label
  • Jitaru, P.; Prete, M.; Cozzi, G.; Turetta, C.; Cairns, W.; Seraglia, R.; Traldi, P.; Cescon, P.; Barbante, C. (2008)
    Speciation analysis of selenoproteins in human serum by solid-phase extraction and affinity HPLC hyphenated to ICP-quadrupole MS
    in Journal of analytical atomic spectrometry (Print)
    (literal)
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  • Jitaru, P.; Prete, M.; Cozzi, G.; Turetta, C.; Cairns, W.; Seraglia, R.; Traldi, P.; Cescon, P.; Barbante, C. (literal)
Pagina inizio
  • 402 (literal)
Pagina fine
  • 406 (literal)
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  • 23 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • DOI: 10.1039/b712693j (literal)
Note
  • ISI Web of Science (WOS) (literal)
Titolo
  • Speciation analysis of selenoproteins in human serum by solid-phase extraction and affinity HPLC hyphenated to ICP-quadrupole MS (literal)
Abstract
  • Anion exchange-solid-phase extraction carried out prior to double column affinity-high performance liquid chromatography (AF-HPLC) coupled on-line with conventional inductively coupled plasma-(quadrupole) mass spectrometry is proposed for simultaneous speciation analysis of selenoprotein P (SelP), glutathione peroxidase (GPx) and selenoalbumin (SeAlb) in human serum. Efficient interference-free AF-HPLC separation of GPx, SelP and SeAlb is obtained within a total chromatographic run time less than 15 min. The repeatability in terms of relative standard deviation (R.S.D., %, n = 10) is 7% for GPx and SelP and 4% for SeAlb. The detection limits are 0.1 ng mL-1 for GPx, 1.0 ng mL-1 for SelP and 1.3 ng mL-1 for SeAlb. The method was validated by the analysis of two human serum reference materials certified for total Se content. (literal)
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