http://www.cnr.it/ontology/cnr/individuo/prodotto/ID169847
Speciation analysis of selenoproteins in human serum by solid-phase extraction and affinity HPLC hyphenated to ICP-quadrupole MS (Articolo in rivista)
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- Label
- Speciation analysis of selenoproteins in human serum by solid-phase extraction and affinity HPLC hyphenated to ICP-quadrupole MS (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Alternative label
Jitaru, P.; Prete, M.; Cozzi, G.; Turetta, C.; Cairns, W.; Seraglia, R.; Traldi, P.; Cescon, P.; Barbante, C. (2008)
Speciation analysis of selenoproteins in human serum by solid-phase extraction and affinity HPLC hyphenated to ICP-quadrupole MS
in Journal of analytical atomic spectrometry (Print)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Jitaru, P.; Prete, M.; Cozzi, G.; Turetta, C.; Cairns, W.; Seraglia, R.; Traldi, P.; Cescon, P.; Barbante, C. (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
- DOI: 10.1039/b712693j (literal)
- Note
- ISI Web of Science (WOS) (literal)
- Titolo
- Speciation analysis of selenoproteins in human serum by solid-phase extraction and affinity HPLC hyphenated to ICP-quadrupole MS (literal)
- Abstract
- Anion exchange-solid-phase extraction carried out prior to double column affinity-high performance liquid chromatography (AF-HPLC) coupled on-line with conventional inductively coupled plasma-(quadrupole) mass spectrometry is proposed for simultaneous speciation analysis of selenoprotein P (SelP), glutathione peroxidase (GPx) and selenoalbumin (SeAlb) in human serum. Efficient interference-free AF-HPLC separation of GPx, SelP and SeAlb is obtained within a total chromatographic run time less than 15 min. The repeatability in terms of relative standard deviation (R.S.D., %, n = 10) is 7% for GPx and SelP and 4% for SeAlb. The detection limits are 0.1 ng mL-1 for GPx, 1.0 ng mL-1 for SelP and 1.3 ng mL-1 for SeAlb. The method was validated by the analysis of two human serum reference materials certified for total Se content. (literal)
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