Engineering of bone using bone marrow stromal cells and a silicon-stabilized tricalcium phosphate bioceramic: evidence for a coupling between bone formation and scaffold resorption (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Engineering of bone using bone marrow stromal cells and a silicon-stabilized tricalcium phosphate bioceramic: evidence for a coupling between bone formation and scaffold resorption (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.biomaterials.2006.10.001 (literal)

Alternative label

• M. Mastrogiacomo, A. Papadimitropoulos, A. Cedola, F. Peyrin, P. Giannoni, S.G. Pearce, M. Alini, C. Giannini. A. Guagliardi and R. Cancedda (2007)
  Engineering of bone using bone marrow stromal cells and a silicon-stabilized tricalcium phosphate bioceramic: evidence for a coupling between bone formation and scaffold resorption
  in Biomaterials
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• M. Mastrogiacomo, A. Papadimitropoulos, A. Cedola, F. Peyrin, P. Giannoni, S.G. Pearce, M. Alini, C. Giannini. A. Guagliardi and R. Cancedda (literal)

Pagina inizio

• 1376 (literal)

Pagina fine

• 1384 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 28 (literal)

Rivista

• Biomaterials (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 7 (literal)

Note

• ISI Web of Science (WOS) (literal)
• Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1- Ist. Naz. per la Ricerca sul Cancro, Univ. di Genova 2- Dip. di Informatica, Sistemistica e Telematica, Univ. di Genova 3- IFN-CNR, Unità di Ancona 4- ESRF, Grenoble, France 5- Biorigen S.r.l., Genova 6- AO Foundation, Davos, Switzerlang 7- IC-CNR, Bari (literal)

Titolo

• Engineering of bone using bone marrow stromal cells and a silicon-stabilized tricalcium phosphate bioceramic: evidence for a coupling between bone formation and scaffold resorption (literal)

Abstract

• Resorbable porous ceramic constructs, based on silicon-stabilized tricalcium phosphate, were implanted in critical-size defects of sheep tibias, either alone or after seeding with bone marrow stromal cells (BMSC). Only BMSC-loaded ceramics displayed a progressive scaffold resorption, coincident with new bone deposition. To investigate the coupled mechanisms of bone formation and scaffold resorption, X-ray computed microtomography (mCT) with synchrotron radiation was performed on BMSC-seeded ceramic cubes. These were analyzed before and after implantation in immunodeficient mice for 2 or 6 months. With increasing implantation time, scaffold thickness significantly decreased while bone thickness increased. The mCT data evidenced that all scaffolds showed a uniform density distribution before implantation. Areas of different segregated densities were instead observed, in the same scaffolds, once seeded with cells and implanted in vivo. A detailed mX-ray diffraction analysis revealed that only in the contact areas between deposited bone and scaffold, the TCP component of the biomaterial decreased much faster than the HA component. This event did not occur at areas away from the bone surface, highlighting coupling and cell-dependency of the resorption and matrix deposition mechanisms. Moreover, in scaffolds implanted without cells, both the ceramic density and the TCP:HA ratio remained unchanged with respect to the preimplantation analysis. (literal)

Prodotto di

• Microscopia a raggi X: sviluppo di ottiche, strumentazione e metodologie per applicazioni biomediche (MD.P03.031.002) (Modulo)
• Istituto di fotonica e nanotecnologie (IFN) (Istituto)

Autore CNR

• ANTONIETTA GUAGLIARDI (Unità di personale interno)
• CINZIA GIANNINI (Persona)
• ALESSIA CEDOLA (Persona)

Insieme di parole chiave

• Parole chiave di "Engineering of bone using bone marrow stromal cells and a silicon-stabilized tricalcium phosphate bioceramic: evidence for a coupling between bone formation and scaffold resorption" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ALESSIA CEDOLA (Persona)
• CINZIA GIANNINI (Persona)
• ANTONIETTA GUAGLIARDI (Unità di personale interno)

Prodotto

• Istituto di fotonica e nanotecnologie (IFN) (Istituto)
• Microscopia a raggi X: sviluppo di ottiche, strumentazione e metodologie per applicazioni biomediche (MD.P03.031.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Biomaterials (Rivista)

Insieme di parole chiave di

• Parole chiave di "Engineering of bone using bone marrow stromal cells and a silicon-stabilized tricalcium phosphate bioceramic: evidence for a coupling between bone formation and scaffold resorption" (Insieme di parole chiave)