http://www.cnr.it/ontology/cnr/individuo/prodotto/ID169199
Solution Structure of Mouse Cripto CFC Domain and Its Inactive Variant Trp107Ala. (Articolo in rivista)
- Type
- Label
- Solution Structure of Mouse Cripto CFC Domain and Its Inactive Variant Trp107Ala. (Articolo in rivista) (literal)
- Anno
- 2006-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1021/jm060772r (literal)
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Calvanese, Luisa; Saporito, Angela; Marasco, Daniela; D'Auria, Gabriella; Minchiotti, Gabriella; Pedone, Carlo; Paolillo, Livio; Falcigno, Lucia; Ruvo, Menotti (2006)
Solution Structure of Mouse Cripto CFC Domain and Its Inactive Variant Trp107Ala.
in Journal of medicinal chemistry
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Calvanese, Luisa; Saporito, Angela; Marasco, Daniela; D'Auria, Gabriella; Minchiotti, Gabriella; Pedone, Carlo; Paolillo, Livio; Falcigno, Lucia; Ruvo, Menotti (literal)
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- 1. CNR, Ist Biostrutture & Bioimmagini, I-80134 Naples, Italy
2. Univ Naples Federico 2, Dipartimento Chim, I-80126 Naples, Italy
3. CNR, Ist Genet & Biofis Adriano B Traverso, I-80126 Naples, Italy (literal)
- Titolo
- Solution Structure of Mouse Cripto CFC Domain and Its Inactive Variant Trp107Ala. (literal)
- Abstract
- We report here for the first time the solution structures at pH 3 and pH 6 of the synthetic CFC domain of mouse Cripto and of the point mutated variant W107A that is unable to bind to the Alk4 Cripto receptor. NMR data confirm that the CFC domain has a C1-C4, C2-C6, C3-C5 disulfide pattern and show that structures are rather flexible and globally extended, with three noncanonical antiparallel strands. His104 and Trp107 side chains protrude from a protein edge and are strongly exposed to solvent, supporting previous evidence of direct involvement in receptor binding. On the opposite molecule side, several nonpolar residues are gathered, forming a large hydrophobic patch that supposedly acts as interface with the cell membrane or the adjacent EGF-like domain. A second hydrophilic patch surrounding His104 and Trp107 is present only in the wild type variant, suggesting a possible involvement in modulating Alk4 recognition. (literal)
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