Recent advances in the assessment of the ratios of cortisol to cortisone and of some of their metabolites in urine by LC-MS-MS (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Recent advances in the assessment of the ratios of cortisol to cortisone and of some of their metabolites in urine by LC-MS-MS (Articolo in rivista) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/jms.1530 (literal)

Alternative label

• Saba A., Raffaelli A., Cupisti A., Petri A., Marcocci C., Salvadori P. (2009)
  Recent advances in the assessment of the ratios of cortisol to cortisone and of some of their metabolites in urine by LC-MS-MS
  in Journal of mass spectrometry (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Saba A., Raffaelli A., Cupisti A., Petri A., Marcocci C., Salvadori P. (literal)

Pagina inizio

• 541 (literal)

Pagina fine

• 548 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 44 (literal)

Rivista

• Journal of mass spectrometry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 8 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CNR-ICCOM Sezione di Pisa; Dipartimento di Chimica e Chimica Industriale, Dipartimento di Medicina Interna, Divisione di Nefrologia, Dipartimento di Endocrinologia e Metabolismo, Ortopedia e Traumatologia, Medicina del Lavoro e AmbiSEN-Environmental and Endocrine and Nervous SystemHigh Technology for the Study of the Effects of Harmful Agents, Università di Pisa (literal)

Titolo

• Recent advances in the assessment of the ratios of cortisol to cortisone and of some of their metabolites in urine by LC-MS-MS (literal)

Abstract

• A previously reported method for the assessment of the ratio of tetrahydrocortisol (THF) + allo-tetrahydrocortisol (A-THF) to tetrahydrocortisone (THE) by HPLC-MS-MS has been significantly improved, in order to increase either ruggedness and reliability. That was achieved by the introduction of an on-line sample cleanup stage, whichmade use of a perfusion column as a solid phase microextraction (SPE) cartridge. The set of analytes was expanded, by introducing cortisol and cortisone, whose ratio supply additional diagnostic information. The response factors of both THF and A-THF has been checked, resulting almost identical, as well as the influence of the matrix on the calibration curves which, although different for water and urine, had similar effect on the ratios of interest. As a consequence, the calibration solutions can be prepared in pure water. The influence of several different storage procedures has also been tested, resulting in no substantial effect on the final result. Finally, the improved method has been used to run real samples fromhealthy volunteers,with satisfactory results. (literal)

Prodotto di

• Istituto di chimica dei composti organo metallici (ICCOM) (Istituto)
• Determinazione di profili metabolici e farmacologici: Sviluppo di metodiche analitiche avanzate per la determinazione quantitativa simultanea di numerosi analiti in fluidi biologici per studi di farmacocinetica e farmacodinamica (PM.P01.005.002) (Modulo)

Autore CNR

• ANDREA RAFFAELLI (Persona)

Insieme di parole chiave

• Keywords of "Recent advances in the assessment of the ratios of cortisol to cortisone and of some of their metabolites in urine by LC-MS-MS" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di chimica dei composti organo metallici (ICCOM) (Istituto)
• Determinazione di profili metabolici e farmacologici: Sviluppo di metodiche analitiche avanzate per la determinazione quantitativa simultanea di numerosi analiti in fluidi biologici per studi di farmacocinetica e farmacodinamica (PM.P01.005.002) (Modulo)

Autore CNR di

• ANDREA RAFFAELLI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of mass spectrometry (Rivista)

Insieme di parole chiave di

• Keywords of "Recent advances in the assessment of the ratios of cortisol to cortisone and of some of their metabolites in urine by LC-MS-MS" (Insieme di parole chiave)