http://www.cnr.it/ontology/cnr/individuo/prodotto/ID168798

Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1 (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1 (Articolo in rivista) (literal)

Anno

2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1074/jbc.M806607200 (literal)

Alternative label

Ponticelli S.; Marasco D.; Tarallo V.; Albuquerque R.J.; Mitola S.; Takeda A.; Stassen J.M.; Presta M.; Ambati J.; Ruvo M.; De Falco S. (2008)
Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1
in The Journal of biological chemistry (Print); American Society Of Biochemistry And Molecular Biology Inc. (ASBMB), Rockville (Stati Uniti d'America)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Ponticelli S.; Marasco D.; Tarallo V.; Albuquerque R.J.; Mitola S.; Takeda A.; Stassen J.M.; Presta M.; Ambati J.; Ruvo M.; De Falco S. (literal)

Pagina inizio

34250 (literal)

Pagina fine

34259 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://www.jbc.org/content/283/49/34250.long (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

283 (literal)

Rivista

?The ?Journal of biological chemistry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

49 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

Pubblicazione su rivista internazionale (literal)

Note

ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Angiogenesis Laboratory and Stem Cell Fate Laboratory, Institute of Genetics and Biophysics Adriano Buzzati-Traverso, Consiglio Nazionale delle Ricerche (CNR), 80131 Napoli, Italy, Institute of Biostructures and Bioimaging, CNR, 80134 Napoli, Italy Department of Ophthalmology and Visual Sciences, University of Kentucky, Lexington, Kentucky 40536, Unit of General Pathology and Immunology, Department of Biomedical Sciences and Biotechnology, University of Brescia, 25123 Brescia, Italy, Thrombogenics, Campus Gasthuisberg, B-3000 Leuven, Belgium (literal)

Titolo

Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1 (literal)

Abstract

Vascular endothelial growth factor receptor-1 (VEGFR-1, also known as Flt-1) is involved in complex biological processes often associated to severe pathological conditions like cancer, inflammation, andmetastasis formation. Consequently, the search for antagonists of Flt-1 has recently gained a growing interest. Here we report the identification of a tetrameric tripeptide from a combinatorial peptide library built using non-natural amino acids, which binds Flt-1 and inhibits in vitro its interaction with placental growth factor (PlGF) and vascular endothelial growth factor (VEGF) A and B (IC50=10µM). The peptide is stable in serum for 7 days and prevents both Flt-1 phosphorylation and the capillarylike tube formation of human primary endothelial cells stimulated by PlGF or VEGF-A. Conversely, the identified peptide does not interfere in VEGF-induced VEGFR-2 activation. In vivo, this peptide inhibits VEGF-A- and PlGF-induced neoangiogenesis in the chicken embryo chorioallantoic membrane assay. In contrast, in the cornea, where avascularity is maintained by high levels of expression of the soluble form of Flt-1 receptor (sFlt-1) that prevents the VEGF-A activity, the peptide is able to stimulate corneal mouse neovascularization in physiological condition, as reported previously for others neutralizing anti-Flt-1 molecules. This tetrameric tripeptide represents a new, promising compound for therapeutic approaches in pathologies where Flt-1 activation plays a crucial role. (literal)

Editore