Structure-function analysis of the EGF-CFC family member Cripto identifies residues essential for nodal signalling (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Structure-function analysis of the EGF-CFC family member Cripto identifies residues essential for nodal signalling (Articolo in rivista) (literal)

Anno

• 2001-01-01T00:00:00+01:00 (literal)

Alternative label

• Minchiotti G., Manco G., Parisi S., Lago C.T., Rosa F., Persico M.G. (2001)
  Structure-function analysis of the EGF-CFC family member Cripto identifies residues essential for nodal signalling
  in Development (Camb.)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Minchiotti G., Manco G., Parisi S., Lago C.T., Rosa F., Persico M.G. (literal)

Pagina inizio

• 4501 (literal)

Pagina fine

• 4510 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 128 (literal)

Rivista

• Development (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Titolo

• Structure-function analysis of the EGF-CFC family member Cripto identifies residues essential for nodal signalling (literal)

Abstract

• Cripto is the founding member of the family of EGF-CFC genes, a class of extracellular factors essential for early vertebrate development. In this study we show that injection of Cripto recombinant protein in mid to late zebrafish Maternal-Zygotic one-eyed pinhead (MZoep) blastulae was able to fully rescue the mutant phenotype, thus providing the first direct evidence that Cripto activity can be added extracellularly to recover oep-encoded function in zebrafish early embryos. Moreover, 15 point mutations and two deletion mutants were generated to assess in vivo their functional relevance by comparing the ability of cripto wild-type and mutant RNAs to rescue the zebrafish MZoep mutant. From this study we concluded that the EGF-CFC domain is sufficient for Cripto biological activity and identified ten point mutations with a functional defective phenotype, two of which, located in the EGF-like domain, correspond to loss-of-function mutations. Finally, we have developed a three-dimensional structural model of Cripto protein and used it as a guide to predict amino acid residues potentially implicated in protein-protein interaction. (literal)

Prodotto di

• Institute of protein biochemistry (IBP) (Istituto)
• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)

Autore CNR

• GABRIELLA MINCHIOTTI (Persona)
• MARIA PERSICO (Persona)
• GIUSEPPE MANCO (Unità di personale interno)
• CARMINE TEODORO LAGO (Unità di personale interno)

Incoming links:

Autore CNR di

• GIUSEPPE MANCO (Unità di personale interno)
• GABRIELLA MINCHIOTTI (Persona)
• CARMINE TEODORO LAGO (Unità di personale interno)
• MARIA PERSICO (Persona)

Prodotto

• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Institute of protein biochemistry (IBP) (Istituto)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Development (Rivista)