Nucleotide-mediated calcium signaling in rat cortical astrocytes: role of P2X and P2Y receptors. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Nucleotide-mediated calcium signaling in rat cortical astrocytes: role of P2X and P2Y receptors. (Articolo in rivista) (literal)

Anno

• 2003-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/glia.10248 (literal)

Alternative label

• Fumagalli M., Brambilla R., D'Ambrosi N., Volontè C., Matteoli M., Verderio C., Abbracchio M.P. (2003)
  Nucleotide-mediated calcium signaling in rat cortical astrocytes: role of P2X and P2Y receptors.
  in GLIA (N.Y.N.Y. : Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Fumagalli M., Brambilla R., D'Ambrosi N., Volontè C., Matteoli M., Verderio C., Abbracchio M.P. (literal)

Pagina inizio

• 218 (literal)

Pagina fine

• 230 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• IF =4.677 SCI-JCR 2003 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 43 (literal)

Rivista

• GLIA (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• I.F. 2002 = 4,193 IF 4,6 - Citazioni 3 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 3 (literal)

Note

• ISI Web of Science (WOS) (literal)
• PubMe (literal)
• Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CNR, FSL, Università di Milano (literal)

Titolo

• Nucleotide-mediated calcium signaling in rat cortical astrocytes: role of P2X and P2Y receptors. (literal)

Abstract

• ATP is the dominant messenger for astrocyte-to-astrocyte calcium-mediated communication. Definition of the exact ATP/P2 receptors in astrocytes and of their coupling to intracellular calcium ([Ca(2+)](i)) has important implications for brain physiology and pathology. We show that, with the only exception of the P2X(6) receptor, primary rat cortical astrocytes express all cloned ligand-gated P2X (i.e., P2X(1-5) and P2X(7)) and G-protein-coupled P2Y receptors (i.e., P2Y(1), P2Y(2), P2Y(4), P2Y(6), and P2Y(12)). These cells also express the P2Y-like UDP-glucose receptor, which has been recently recognized as the P2Y(14) receptor. Single-cell image analysis showed that only some of these receptors are coupled to [Ca(2+)](i). While ATP induced rapid and transient [Ca(2+)](i) increases (counteracted by the P2 antagonists suramin, pyridoxal-phosphate-6-azophenyl-2'-4'-disulfonic acid and oxidized ATP), the P2X(1)/P2X(3) agonist alphabetameATP produced no changes. Conversely, the P2X(7) agonist BzATP markedly increased [Ca(2+)](i); the presence and function of the P2X(7) receptor was also confirmed by the formation of the P2X(7) pore. ADP and 2meSADP also produced [Ca(2+)](i) increases antagonized by the P2Y(1) antagonist MRS2179. Some cells also responded to UTP but not to UDP. Significant responses to sugar-nucleotides were also detected, which represents the first functional response reported for the putative P2Y(14) receptor in a native system. Based on agonist preference of known P2 receptors, we conclude that, in rat astrocytes, ATP-induced calcium rises are at least mediated by P2X(7) and P2Y(1) receptors; additional receptors (i.e., P2X(2), P2X(4), P2X(5), P2Y(2), P2Y(4), and P2Y(14)) may also contribute. Copyright 2003 Wiley-Liss, Inc. (literal)

Prodotto di

• Istituto di neuroscienze (IN) (Istituto)
• Istituto di neurobiologia e medicina molecolare (INMM) (Istituto)

Autore CNR

• CLAUDIA VERDERIO (Unità di personale interno)
• CINZIA VOLONTE' (Unità di personale interno)

Incoming links:

Autore CNR di

• CINZIA VOLONTE' (Unità di personale interno)
• CLAUDIA VERDERIO (Unità di personale interno)

Prodotto

• Istituto di neuroscienze (IN) (Istituto)
• Istituto di neurobiologia e medicina molecolare (INMM) (Istituto)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• GLIA (Rivista)