Determination and characterization of phytochelatins by liquid chromatography coupled with on line chemical vapour generation and atomic fluorescence spectrometric detection. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Determination and characterization of phytochelatins by liquid chromatography coupled with on line chemical vapour generation and atomic fluorescence spectrometric detection. (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.chroma.2006.08.045 (literal)

Alternative label

• Bramanti E.; Toncelli D.; Morelli E.; Lampugnani L.; Zamboni R.; Miller K.E.; Zemetra J.; D'Ulivo A. (2006)
  Determination and characterization of phytochelatins by liquid chromatography coupled with on line chemical vapour generation and atomic fluorescence spectrometric detection.
  in Journal of chromatography (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Bramanti E.; Toncelli D.; Morelli E.; Lampugnani L.; Zamboni R.; Miller K.E.; Zemetra J.; D'Ulivo A. (literal)

Pagina inizio

• 195 (literal)

Pagina fine

• 203 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 1133 (literal)

Rivista

• Journal of chromatography (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Emilia Bramanti, Leonardo Lampugnani, Alessandro D'Ulivo - Istituto per i Processi Chimico-Fisici (CNR) , Laboratory of Instrumental Analytical Chemistry, Via G. Moruzzi 1, 56124- Pisa, Italy; Daniel Toncelli, Roberto Zamboni - Department of Chemistry and Industrial Chemistry, University of Pisa, Via Risorgimento 35, 56126 Pisa, Italy; Elisabetta Morelli - Istituto di Biofisica (CNR), Via G. Moruzzi 1, 56124- Pisa, Italy; Keith E. Miller, Joseph Zemetra - Department of Chemistry and Biochemistry, University of Denver, Denver, CO 80208, USA (literal)

Titolo

• Determination and characterization of phytochelatins by liquid chromatography coupled with on line chemical vapour generation and atomic fluorescence spectrometric detection. (literal)

Abstract

• Liquid chromatography (LC) coupled on line with UV/visible diode array detector (DAD) and cold vapour generation atomic fluorescence spectrometry (CVGAFS) has been developed for the speciation, determination and characterization of phytochelatins (PCs). The method is based on a bidimensional approach, e.g. on the analysis of synthetic PC solutions (apo-PCs and Cd(2+)-complexed PCs) (i) by size exclusion chromatography coupled to UV diode array detector (SEC-DAD); (ii) by the derivatization of PC -SH groups in SEC fractions by p-hydroxymercurybenzoate (PHMB) and the indirect detection of PC-PHMB complexes by reversed phase liquid chromatography coupled to atomic fluorescence detector (RPLC-CVGAFS). MALDI-TOF/MS (matrix assisted laser desorption ionization time of flight mass spectrometry) analysis of underivatized synthetic PC samples was performed in order have a qualitative information of their composition. Quantitative analysis of synthetic PC solutions has been performed on the basis of peak area of PC-PHMB complexes of the mercury specific chromatogram and calibration curve of standard solution of glutathione (GSH) complexed to PHMB (GS-PHMB). The limit of quantitation (LOQ) in terms of GS-PHMB complex was 90 nM (CV 5%) with an injection volume of 35 microL, corresponding to 3.2 pmol (0.97 ng) of GSH. The method has been applied to analysis of extracts of cell cultures from Phaeodactylum tricornutum grown in Cd-containing nutrient solutions, analysed by SEC-DAD-CVGAFS and RPLC-DAD-CVGAFS. (literal)

Prodotto di

• Meccanismi di risposta cellulare a contaminanti ambientali (TA.P05.015.002) (Modulo)
• Istituto di biofisica (IBF) (Istituto)
• Istituto per i processi chimico-fisici (IPCF) (Istituto)
• Termodinamica e caratterizzazione dei materiali (MD.P01.010.001) (Modulo)

Autore CNR

• ELISABETTA MORELLI (Unità di personale interno)
• LEONARDO LAMPUGNANI (Unità di personale interno)
• EMILIA BRAMANTI (Persona)
• ALESSANDRO D'ULIVO (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Determination and characterization of phytochelatins by liquid chromatography coupled with on line chemical vapour generation and atomic fluorescence spectrometric detection." (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto per i processi chimico-fisici (IPCF) (Istituto)
• Istituto di biofisica (IBF) (Istituto)
• Termodinamica e caratterizzazione dei materiali (MD.P01.010.001) (Modulo)
• Meccanismi di risposta cellulare a contaminanti ambientali (TA.P05.015.002) (Modulo)

Autore CNR di

• ALESSANDRO D'ULIVO (Unità di personale interno)
• EMILIA BRAMANTI (Persona)
• ELISABETTA MORELLI (Unità di personale interno)
• LEONARDO LAMPUGNANI (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of chromatography (Rivista)

Insieme di parole chiave di

• Parole chiave di "Determination and characterization of phytochelatins by liquid chromatography coupled with on line chemical vapour generation and atomic fluorescence spectrometric detection." (Insieme di parole chiave)