Uptake and recycling of pro-BDNF for transmitter-induced secretion by cortical astrocytes. (Articolo in rivista)

Type
Label
  • Uptake and recycling of pro-BDNF for transmitter-induced secretion by cortical astrocytes. (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Alternative label
  • Bergami M, Santi S, Formaggio E, Cagnoli C, Verderio C, Blum R, Berninger B, Matteoli M, Canossa M. (2008)
    Uptake and recycling of pro-BDNF for transmitter-induced secretion by cortical astrocytes.
    in The Journal of cell biology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Bergami M, Santi S, Formaggio E, Cagnoli C, Verderio C, Blum R, Berninger B, Matteoli M, Canossa M. (literal)
Pagina inizio
  • 213 (literal)
Pagina fine
  • 221 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 183 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Department of Human and General Physiology and 2 Department of Pharmacology, University of Bologna, I-40126 Bologna, Italy; Istituto di Genetica Molecolare del Consiglio Nazionale delle Ricerche e Istituti Ortopedici Rizzoli, I-40136 Bologna, Italy; Department of Medicine and Public Health, University of Verona, I-37134 Verona, Italy; Dipartimento di Farmacologia, Istituto di Neuroscienze del Consiglio Nazionale delle Ricerche, Università di Milano, I-20129 Milano, Italy; Department of Physiological Genomics, Institute of Physiology, Ludwig-Maximilians University Munich, D-80336 Munich, Germany. Institute of Stem Cell Research, Helmholtz Zentrum Muenchen, German Research Center for Environmental Health, D-85764 Neuherberg, Germany; Istituto di Ricovero e Cura a Carattere Scientifico Don Gnocchi, I-20148 Milano, Italy; Italian Institute of Technology, I-16163 Genoa, Italy (literal)
Titolo
  • Uptake and recycling of pro-BDNF for transmitter-induced secretion by cortical astrocytes. (literal)
Abstract
  • Activity-dependent secretion of brain-derived neurotrophic factor (BDNF) is thought to enhance synaptic plasticity, but the mechanisms controlling extracellular availability and clearance of secreted BDNF are poorly understood. We show that BDNF is secreted in its precursor form (pro-BDNF) and is then cleared from the extracellular space through rapid uptake by nearby astrocytes after theta-burst stimulation in layer II/III of cortical slices, a paradigm resulting in long-term potentiation of synaptic transmission. Internalization of pro-BDNF occurs via the formation of a complex with the pan-neurotrophin receptor p75 and subsequent clathrin-dependent endocytosis. Fluorescence-tagged pro-BDNF and real-time total internal reflection fluorescence microscopy in cultured astrocytes is used to monitor single endocytic vesicles in response to the neurotransmitter glutamate. We find that endocytosed pro-BDNF is routed into a fast recycling pathway for subsequent soluble NSF attachment protein receptor-dependent secretion. Thus, astrocytes contain an endocytic compartment competent for pro-BDNF recycling, suggesting a specialized form of bidirectional communication between neurons and glia. (literal)
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