Proliferation and activation of bronchial epithelial cells in corticosteroid-dependent asthma. (Articolo in rivista)

Type
Label
  • Proliferation and activation of bronchial epithelial cells in corticosteroid-dependent asthma. (Articolo in rivista) (literal)
Anno
  • 2001-01-01T00:00:00+01:00 (literal)
Alternative label
  • 1.Vignola AM, 2.Chiappara G, 2.Siena L, 2.Bruno A, 2.Gagliardo R, 3.Merendino AM, 4.Polla BS, 5.Arrigo AP, 2.Bonsignore G, 5.Bousquet J, 6.Chanez P. (2001)
    Proliferation and activation of bronchial epithelial cells in corticosteroid-dependent asthma.
    in Journal of allergy and clinical immunology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • 1.Vignola AM, 2.Chiappara G, 2.Siena L, 2.Bruno A, 2.Gagliardo R, 3.Merendino AM, 4.Polla BS, 5.Arrigo AP, 2.Bonsignore G, 5.Bousquet J, 6.Chanez P. (literal)
Pagina inizio
  • 738 (literal)
Pagina fine
  • 746 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 108 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • Impact Factor: 5.560 PubMed PMID: 11692098. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Lo studio dimostra che in soggetti asmatici steroido-dipendenti, i markers di sopravvivenza delle cellule epiteliali bronchiali sono espressi in parallelo con i markers di attivazione. Esso suggerisce che i fenomeni di riparazione dell'epitelio sono presenti in contemporanea con i fenomeni di attivazione. (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1. Istituto di Medicina Generale e Pneumologia - Responsabile Sezione IFC-IBIM-CNR.2.IBIM-CNR.3.Istituto di Medicina Generale e Pneumologia-Università degli Studi di Palermo. 4. Laboratoire de Physiologie Respiratoire, UFR Cochin Port-Royal, Université Paris.5. Laboratoire du Stress Cellulaire, CGMC, CNRS UMR 5534, Université Claude Bernard, Lyon 6.INSERM-Montpellier Francia. (literal)
Titolo
  • Proliferation and activation of bronchial epithelial cells in corticosteroid-dependent asthma. (literal)
Abstract
  • BACKGROUND: Structural and functional characteristics of bronchial epithelial cells in corticosteroid-dependent asthma are unknown. OBJECTIVE: In bronchial biopsy specimens from 16 control, 9 untreated asthmatic, 9 inhaled corticosteroid-treated asthmatic, and 19 corticosteroid-dependent asthmatic subjects, we evaluated epithelium morphology and patterns of cell apoptosis, proliferation, and activation. METHODS: We used the terminal deoxynucleotidyl-mediated dUTP nick end labeling (TUNEL) technique to study apoptosis. Immunohistochemistry was used to evaluate the expression of molecules related to apoptosis (such as Bcl-2 and P53), cell proliferation (PCNA), and cell activation (NFkappaB and CD40/CD40-L). RESULTS: Epithelium thickness was higher in corticosteroid-dependent asthmatic and control subjects than in inhaled corticosteroid-treated and untreated asthmatic subjects (P < .0001 and P <.0003). Very few TUNEL-positive epithelial cells were found in the 4 groups. Bcl-2 expression was higher in all groups of asthmatic subjects than in controls (P < .001). In corticosteroid-dependent asthmatic subjects, PCNA, NFkappaB, and CD40-L expression was higher than in inhaled corticosteroid-treated asthmatic (P < .001), untreated asthmatic (P <.001 and P < .04), and control (P < .01) subjects. CD40 expression was greater in corticosteroid-dependent asthmatic and untreated asthmatic subjects than in inhaled corticosteroid-treated asthmatic subjects (P < .0001 and P < .0006) and controls (P < .02 and P < .03). In corticosteroid-dependent asthma, PCNA expression was correlated with the epithelium thickness (P < .007). CONCLUSION: This study shows that in bronchial epithelial cells of corticosteroid-dependent asthma, markers of cell survival and proliferation are coexpressed with markers of cell activation, suggesting that in this disease epithelium repair is associated with a persistent activation state of epithelial cells. (literal)
Prodotto di
Autore CNR

Incoming links:


Autore CNR di
Prodotto
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
data.CNR.it