Identification of the minimal melanocyte-specific promoter in the melanocortin receptor 1 gene (Articolo in rivista)

Type
Label
  • Identification of the minimal melanocyte-specific promoter in the melanocortin receptor 1 gene (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1186/1756-9966-27-71 (literal)
Alternative label
  • Miccadei S. 1; Pascucci B. 2,3; Picardo M. 4; Natali P.G. 1; Civitareale D. 2 (2008)
    Identification of the minimal melanocyte-specific promoter in the melanocortin receptor 1 gene
    in Journal of experimental & clinical cancer research (Online)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Miccadei S. 1; Pascucci B. 2,3; Picardo M. 4; Natali P.G. 1; Civitareale D. 2 (literal)
Pagina inizio
  • 71 (literal)
Pagina fine
  • 79 (literal)
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  • Rivista Open Access. (literal)
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  • 27 (literal)
Rivista
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  • Epub ahead of print doi:10.1186/1756-9966-27-71 (literal)
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  • 9 (literal)
Note
  • ISI Web of Science (WOS) (literal)
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  • 1 = Laboratory of Molecular Pathology and Ultrastructure, Regina Elena Cancer Institute, Via delle Messi d'Oro 156, 00158 Rome, Italy; 2 = Institute of Neurobiology and Molecular Medicine, CNR, Via Fosso del Cavaliere,100,00133 Rome, Italy; 3 = Institute of Cristallography, CNR, Via Salaria, 00016 Monterotondo Stazione, Italy; 4 = Laboratory of Cutaneous Physiopathology, San Gallicano Dermatological Institute, Via San Gallicano 25/a,00153 Rome, Italy (literal)
Titolo
  • Identification of the minimal melanocyte-specific promoter in the melanocortin receptor 1 gene (literal)
Abstract
  • ABSTRACT: BACKGROUND: The understanding of cutaneous pigmentation biology is relevant from the biologic and clinical point of view. The binding of alpha-melanocortin and its specific receptor, on the plasma membrane of melanin synthetizing cells, plays a crucial role in melanins biosynthesis. Furthermore, loss of MC1R function is associated with an increased incidence of melanoma and non-melanoma skin cancer. The expression of the alpha-melanocortin receptor gene is highly controlled but, at the present , region responsible for tissue-specific activity of the gene promoter has not been identified. METHODS: We have cloned the genomic sequences upstream the human MC1R coding gene. A DNA fragment of 5 kilobases upstream the human MC1R encoding sequence was placed in front of a reporter gene and several deletion mutants of such fragment have been prepared. These constructs have been tested for the ability to drive the melanocyte-specific gene expression of the reporter gene using transfection experiments in melanocyte and non-melanocyte cell lines. From these experiments we identified a DNA fragment with the ability to drive the gene transcription in a tissue-specific way and we used this small DNA fragment in DNA-protein interaction assays. RESULTS: We show that the 150 base pairs upstream the MC1R gene initiation codon are able to drive the melanocyte-specific gene transcription. Furthermore, we provide experimental evidences suggesting that on such minimal melanocyte-specific gene promoter can assemble tissue-specific complexes. CONCLUSIONS: The present results strongly imply that the transcriptional regulation of the melanocyte-specific MC1R gene requires an internal promoter located in the 150 base pairs upstream the initiation codon. (literal)
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