Beta-sitosterol among other secondary metabolites of Piper galeatum shows inhibition of TNF alpha-induced cell adhesion molecule expression on human endothelial cells (Articolo in rivista)

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  • Beta-sitosterol among other secondary metabolites of Piper galeatum shows inhibition of TNF alpha-induced cell adhesion molecule expression on human endothelial cells (Articolo in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.biochi.2010.06.005 (literal)
Alternative label
  • Gupta P.; Balwani S.; Kumar S.; Aggarwal N.; Rossi M.; Paumier S.; Caruso F.; Bovicelli P.; Saso L,; DePass A.L.; Prasad A.K.; Parmar V.S.; Ghosh B. (2010)
    Beta-sitosterol among other secondary metabolites of Piper galeatum shows inhibition of TNF alpha-induced cell adhesion molecule expression on human endothelial cells
    in Biochimie (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Gupta P.; Balwani S.; Kumar S.; Aggarwal N.; Rossi M.; Paumier S.; Caruso F.; Bovicelli P.; Saso L,; DePass A.L.; Prasad A.K.; Parmar V.S.; Ghosh B. (literal)
Pagina inizio
  • 1213 (literal)
Pagina fine
  • 1221 (literal)
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  • 92 (literal)
Rivista
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  • 9 (literal)
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  • Scopu (literal)
  • ISI Web of Science (WOS) (literal)
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  • a Bioorganic Laboratory, Department of Chemistry, University of Delhi, Delhi-110 007, India; b Molecular Immunogenetics Laboratory, Institute of Genomics and Integrative Biology (IGIB), Mall Road, Delhi University Campus, Delhi-110 007, India; c Vassar College, Department of Chemistry, Poughkeepsie, NY 12604-0484, USA; d Instituto di Chimica Biomolecolare, CNR, Piazzale Aldo Moro 5, Rome 00185, Italy; e Department of Physiology and Pharmacology 'Vittorio Erspamer', Sapienza University, Piazzale Aldo Moro 5, Rome 00185, Italy; f Department of Biology, Long Island University-Brooklyn, 1 University Plaza, Brooklyn, NY 11201, USA. (literal)
Titolo
  • Beta-sitosterol among other secondary metabolites of Piper galeatum shows inhibition of TNF alpha-induced cell adhesion molecule expression on human endothelial cells (literal)
Abstract
  • A phytochemical investigation of the stems of Piper galeatum yielded one novel amide, 1-(3'-hydroxy-5'-methoxycinnamoyl)-piperidine (5) along with four known compounds, i.e. beta-sitosterol (1), cyclostachine-A (2), piperine (3) and piperolein-B (4). The structures of all the five compounds, isolated for the first time from this plant were unambiguously established on the basis of their detailed spectral analysis. The structure of cyclostachine-A (2) was confirmed by X-ray crystallographic studies and structures of known compounds were confirmed by comparison of their physical and/or chemical data with those reported in the literature, which were in complete agreement. Additionally, the crude extracts as well as the isolated pure compounds were screened for their activity to inhibit TNF alpha (tumour necrosis factor-alpha)-induced expression of cell adhesion molecule ICAM-1 (intercellular adhesion molecule-1) on the surface of human umbilical vein endothelial cells (HUVECs). Among all, beta-sitosterol (1) was found to be the most active compound, which was taken for further studies. beta-sitosterol also significantly inhibited the TNF alpha-induced expression of VCAM-1 and E-selectin, which also play key role in various inflammatory diseases. The functional correlation of cell adhesion molecules inhibition was assessed by cell adhesion assay using human neutrophils. We found that beta-sitosterol significantly blocks the adhesion of neutrophils to endothelial monolayer. To elucidate the molecular mechanism of inhibition of cell adhesion molecules, we investigated the status of nuclear transcription factor-kappa B (NF-kappa B) and were able to establish that beta-sitosterol significantly blocked the TNF alpha-induced activation of NF-kappa B. (C) 2010 Elsevier Masson SAS. All rights reserved. (literal)
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