http://www.cnr.it/ontology/cnr/individuo/prodotto/ID15679
Fractional volume integration in two-dimensional NMR spectra: CAKE, a Monte Carlo approach (Articolo in rivista)
- Type
- Label
- Fractional volume integration in two-dimensional NMR spectra: CAKE, a Monte Carlo approach (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.jmr.2008.03.010 (literal)
- Alternative label
Romano R.; Paris D.; Acernese F.; Barone F.; Motta A.; (2008)
Fractional volume integration in two-dimensional NMR spectra: CAKE, a Monte Carlo approach
in Journal of magnetic resonance (San Diego, Calif., 1997 : Print)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Romano R.; Paris D.; Acernese F.; Barone F.; Motta A.; (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Dipartimento di Scienze Farmaceutiche, Università degli Studi di Salerno, Via Ponte Don Melillo, I-84084 Fisciano, Salerno, Italy;
Istituto di Chimica Biomolecolare del CNR, Comprensorio Olivetti, Edificio A, Via Campi Flegrei 34, I-80078 Pozzuoli, Naples, Italy. (literal)
- Titolo
- Fractional volume integration in two-dimensional NMR spectra: CAKE, a Monte Carlo approach (literal)
- Abstract
- Quantitative information from multi-dimensional NMR experiments can be obtained by peak volume
integration. The standard procedure (selection of a region around the chosen peak and addition of all values)
is often biased by poor peak definition because of peak overlap. Here we describe a simple method,
called CAKE, for volume integration of (partially) overlapping peaks. Assuming the axial symmetry of
two-dimensional NMR peaks, as it occurs in NOESY and TOCSY when Lorentz-Gauss transformation of
the signals is carried out, CAKE estimates the peak volume by multiplying a volume fraction by a factor
R. It represents a proportionality ratio between the total and the fractional volume, which is identified as
a slice in an exposed region of the overlapping peaks. The volume fraction is obtained via Monte Carlo
Hit-or-Miss technique, which proved to be the most efficient because of the small region and the limited
number of points within the selected area. Tests on simulated and experimental peaks, with different
degrees of overlap and signal-to-noise ratios, show that CAKE results in improved volume estimates. A
main advantage of CAKE is that the volume fraction can be flexibly chosen so as to minimize the effect
of overlap, frequently observed in two-dimensional spectra. (literal)
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