NMR studies of flexible peptides in cavities mimicking the synaptic cleft (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• NMR studies of flexible peptides in cavities mimicking the synaptic cleft (Articolo in rivista) (literal)

Anno

• 2002-01-01T00:00:00+01:00 (literal)

Alternative label

• Pavone L. 1, Crescenzi O. 2, Tancredi T. 3, Temussi P.A. 4 (2002)
  NMR studies of flexible peptides in cavities mimicking the synaptic cleft
  in FEBS letters (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Pavone L. 1, Crescenzi O. 2, Tancredi T. 3, Temussi P.A. 4 (literal)

Pagina inizio

• 273 (literal)

Pagina fine

• 276 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• ISSN: 0014-5793. IF 3.912 (2002) Citations 53988 (2002) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 513 (literal)

Rivista

• FEBS letters (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• Pavone L.: Laureando UNI Napoli (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• The work demonstrates that: i) it is possible to run NMR spectra in a confined environment mimicking the synaptic cleft, ii) the mere confinement is sufficient to induce a change in viscosity of the aqueous solution without changing the composition and the temperature of the solvent, iii) NOESY spectra hint either a conformational selection or a slowing down of internal motions: both phenomena can favor the interaction of the peptide with the receptor. The finding that the very geometry of the site is sufficient to induce a substantial increase in viscosity and the ensuing ordering effect on flexible neurotransmitters shows the importance of performing structural studies in environments that mimic the natural ones as closely as possible. (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1 UNI Napoli \"Federico II\", 2 UNI Napoli \"Federico II\", 3 ICB Napoli, 4 UNI Napoli \"Federico II\" (literal)

Titolo

• NMR studies of flexible peptides in cavities mimicking the synaptic cleft (literal)

Abstract

• Interaction of neuropeptides with postsynaptic receptors is characterized by a high entropic barrier originating from the combination of nanomolar concentration with low conformer population. The influence of high viscosity environments on conformer distribution can help overcoming this difficulty. In the attempt of simulating the physicochemical conditions of the synaptic cleft, 15N-labeled enkephalin has been studied in polyacrilamide gels swollen by different aqueous solutions in the temperature range 273-293 K. NOESY spectra in the gel pores are consistent with a conformational selection or a slowing down of internal motions that can favor the interaction of the peptide with the receptor. (literal)

Prodotto di

• Istituto di chimica biomolecolare (ICB) (Istituto)

Autore CNR

• TEODORICO TANCREDI (Persona)

Insieme di parole chiave

• Keywords of "NMR studies of flexible peptides in cavities mimicking the synaptic cleft" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• TEODORICO TANCREDI (Persona)

Prodotto

• Istituto di chimica biomolecolare (ICB) (Istituto)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• FEBS letters (Rivista)

Insieme di parole chiave di

• Keywords of "NMR studies of flexible peptides in cavities mimicking the synaptic cleft" (Insieme di parole chiave)