An endogenous cannabinoid tone attenuates cholera toxin-induced fluid accumulation in mice. (Articolo in rivista)

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Label
  • An endogenous cannabinoid tone attenuates cholera toxin-induced fluid accumulation in mice. (Articolo in rivista) (literal)
Anno
  • 2003-01-01T00:00:00+01:00 (literal)
Alternative label
  • Izzo AA, Capasso F, Costagliola A, Bisogno T, Marsicano G, Ligresti A, Matias I, Capasso R, Pinto L, Borrelli F, Cecio A, Lutz B, Mascolo N, Di Marzo V. (2003)
    An endogenous cannabinoid tone attenuates cholera toxin-induced fluid accumulation in mice.
    in Gastroenterology (N.Y.N.Y., 1943)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Izzo AA, Capasso F, Costagliola A, Bisogno T, Marsicano G, Ligresti A, Matias I, Capasso R, Pinto L, Borrelli F, Cecio A, Lutz B, Mascolo N, Di Marzo V. (literal)
Pagina inizio
  • 765 (literal)
Pagina fine
  • 774 (literal)
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  • 125 (literal)
Rivista
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  • Ligresti A Matias I assegniste ICB (literal)
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  • Per la prima volta si riporta il coinvolgimento del sistema cannabinoide endogeno nella ipersecrezione intestinale indotta dalla tossina del colera. Questa evidenza sperimentale, che prevede una over-stimolazione del sistema endocannabinoide in grado di svolgere un ruolo antisecretorio, protettivo nell’intestino, è in perfetto accordo con il numero sempre più crescente di pubblicazioni scientifiche che correlano il sistema endocannabinoide a particolari condizioni patologiche. (literal)
Note
  • ISI Web of Science (WOS) (literal)
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  • Department of Experimental Pharmacology, Department of Biological Structure, Functions and Technology, University of Naples, \"Frederico II\", Naples; Endocannabinoid Research Group, Institutes of Biomolecular Chemistry and Cybernetics, National Research Council, 80078 Pozzuoli, Napoli, Italy; Molecular Genetics of Behaviour, Max Planck Institute of Psychiatry, Munich, Germany; Department of Pharmaceutical Science, Fisciano, Salerno, Italy (literal)
Titolo
  • An endogenous cannabinoid tone attenuates cholera toxin-induced fluid accumulation in mice. (literal)
Abstract
  • BACKGROUND & AIMS: Cholera toxin (CT) is the most recognizable enterotoxin causing secretory diarrhea, a major cause of infant morbidity and mortality throughout the world. In this study, we investigated the role of the endogenous cannabinoid system (i.e., the cannabinoid receptors and their endogenous ligands) in CT-induced fluid accumulation in the mouse small intestine. METHODS: Fluid accumulation was evaluated by enteropooling; endocannabinoid levels were measured by isotope-dilution gas chromatography mass spectrometry; CB(1) receptors were localized by immunohistochemistry and their messenger RNA (mRNA) levels were quantified by reverse-transcription polymerase chain reaction (PCR). RESULTS: Oral administration of CT to mice resulted in an increase in fluid accumulation in the small intestine and in increased levels of the endogenous cannabinoid, anandamide, and increased expression of the cannabinoid CB(1) receptor mRNA. The cannabinoid receptor agonist CP55,940 and the selective cannabinoid CB(1) receptor agonist arachidonoyl-chloro-ethanolamide inhibited CT-induced fluid accumulation, and this effect was counteracted by the CB(1) receptor antagonist SR141716A, but not by the CB(2) receptor antagonist SR144528. SR141716A, per se, but not the vanilloid VR1 receptor antagonist capsazepine, enhanced fluid accumulation induced by CT, whereas the selective inhibitor of anandamide cellular uptake, VDM11, prevented CT-induced fluid accumulation. CONCLUSIONS: These results indicate that CT, along with enhanced intestinal secretion, causes overstimulation of endocannabinoid signaling with an antisecretory role in the small intestine. (literal)
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