Exofacial protein thiols as a route for the internalization of Gd(III)-based complexes for magnetic resonance imaging cell labeling (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Exofacial protein thiols as a route for the internalization of Gd(III)-based complexes for magnetic resonance imaging cell labeling (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1021/jm901876r (literal)

Alternative label

• Digilio G; Menchise V; Gianolio E; Catanzaro V; Carrera C; Napolitano R; Fedeli F, Aime S. (2010)
  Exofacial protein thiols as a route for the internalization of Gd(III)-based complexes for magnetic resonance imaging cell labeling
  in Journal of medicinal chemistry
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Digilio G; Menchise V; Gianolio E; Catanzaro V; Carrera C; Napolitano R; Fedeli F, Aime S. (literal)

Pagina inizio

• 4877 (literal)

Pagina fine

• 4890 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 53 (literal)

Rivista

• Journal of medicinal chemistry (Rivista)

Note

• Scopu (literal)
• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Department of Environmental and Life Sciences, University of Eastern Piedmont \"A. Avogadro\", Viale T. Michel 11, I-15121 Alessandria, Italy; Institute for Biostructures and Bioimages (CNR), c/o Molecular Biotechnology Center, University of Turin, Via Nizza 52, I-10125 Torino, Italy; Department of Chemisty, IFM and Center for Molecular Imaging, University of Turin, Via Nizza 52, I-10125 Torino, Italy (literal)

Titolo

• Exofacial protein thiols as a route for the internalization of Gd(III)-based complexes for magnetic resonance imaging cell labeling (literal)

Abstract

• Four novel MRIGd(III)-based probes have been synthesized and evaluated for their labeling properties on cultured cell lines K562, C6, and B16. The labeling strategy relies upon the fact that cells display a large number of reactive exofacial protein thiols (EPTs) that can be exploited as anchorage points for suitably activated MRI probes. The probes are composed of a Gd(III) chelate (based on eitherDO3A or DTPA) connected through a flexible linker to the 2-pyridyldithio chemical function for binding to EPTs. GdDO3A-based chelates could efficiently label cells (up to a level of 1.2 ? 1010 Gd(III) atoms/cell), whereas GdDTPA-based chelates showed poor or no cell labeling ability at all. Among the GdDO3A based compounds, that having the longest spacer (compound GdL1A) showed the best labeling efficacy. The mechanism of EPT mediated cell labeling by GdL1A involves probe internalization without sequestration of the Gd(III) chelate within subcellular structures such as endosomes. (literal)

Prodotto di

• Design di sonde paramagnetiche per lo sviluppo di protocolli diagnostici con tecniche MRI (PM.P01.003.004) (Modulo)
• Institute of biostructure and bioimaging (IBB) (Istituto)

Autore CNR

• VALERIA MENCHISE (Persona)

Insieme di parole chiave

• Parole chiave di "Exofacial protein thiols as a route for the internalization of Gd(III)-based complexes for magnetic resonance imaging cell labeling" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of biostructure and bioimaging (IBB) (Istituto)
• Design di sonde paramagnetiche per lo sviluppo di protocolli diagnostici con tecniche MRI (PM.P01.003.004) (Modulo)

Autore CNR di

• VALERIA MENCHISE (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of medicinal chemistry (Rivista)

Insieme di parole chiave di

• Parole chiave di "Exofacial protein thiols as a route for the internalization of Gd(III)-based complexes for magnetic resonance imaging cell labeling" (Insieme di parole chiave)