The Metal Loading Ability of ²-Amyloid N-Terminus. A Combined Potentiometric and Spectroscopic Study of Copper(II) Complexes with ²-Amyloid(1-16), its Short or Mutated Peptide Fragments and its PEG-ylated Analogue (Articolo in rivista)

Type
Label
  • The Metal Loading Ability of ²-Amyloid N-Terminus. A Combined Potentiometric and Spectroscopic Study of Copper(II) Complexes with ²-Amyloid(1-16), its Short or Mutated Peptide Fragments and its PEG-ylated Analogue (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1021/ic8006052 (literal)
Alternative label
  • Chiara A. Damante; Katalin Osz, Zoltán Nagy; Giuseppe Pappalardo; Giulia Grasso; Giuseppe Impellizzeri; Enrico Rizzarelli; Imre Sóvágó. (2008)
    The Metal Loading Ability of ²-Amyloid N-Terminus. A Combined Potentiometric and Spectroscopic Study of Copper(II) Complexes with ²-Amyloid(1-16), its Short or Mutated Peptide Fragments and its PEG-ylated Analogue
    in Inorganic chemistry; ACS, American chemical society, Washington, DC (Stati Uniti d'America)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Chiara A. Damante; Katalin Osz, Zoltán Nagy; Giuseppe Pappalardo; Giulia Grasso; Giuseppe Impellizzeri; Enrico Rizzarelli; Imre Sóvágó. (literal)
Pagina inizio
  • 9669 (literal)
Pagina fine
  • 9683 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 47 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Department of Chemical Sciences, UniVersity of Catania, V.le A. Doria 6, 95125 Catania, Italy, Department of Inorganic and Analytical Chemistry, UniVersity of Debrecen, 4010 Debrecen, Hungary, and CNR Institute of Biostructures and Bioimaging, V.le A. Doria, 95125 Catania, Italy (literal)
Titolo
  • The Metal Loading Ability of ²-Amyloid N-Terminus. A Combined Potentiometric and Spectroscopic Study of Copper(II) Complexes with ²-Amyloid(1-16), its Short or Mutated Peptide Fragments and its PEG-ylated Analogue (literal)
Abstract
  • Alzheimer's disease (AD) is becoming a rapidly growing health problem, as it is one of the main causes of dementia in the elderly. Interestingly, copper(II) (together with zinc and iron) ions are accumulated in amyloid deposits, suggesting that metal binding to A? could be involved in AD pathogenesis. In A?, the metal binding is believed to occur within the N-terminal region encompassing the amino acid residues 1-16. In this work, potentiometric, spectroscopic (UV-vis, circular dichroism, and electron paramagnetic resonance), and electrospray ionization mass spectrometry (ESI-MS) approaches were used to investigate the copper(II) coordination features of a new polyethylene glycol (PEG)-conjugated A? peptide fragment encompassing the 1-16 amino acid residues of the N-terminal region (A?(1-16)PEG). The high water solubility of the resulting metal complexes allowed us to obtain a complete complex speciation at different metal-to-ligand ratios ranging from 1:1 to 4:1. Potentiometric and ESI-MS data indicate that A?(1-16)PEG is able to bind up to four copper(II) ions. Furthermore, in order to establish the coordination environment at each metal binding site, a series of shorter peptide fragments of A?, namely, A?(1-4), A?(1-6), AcA?(1-6), and AcA?(8-16)Y10A, were synthesized, each encompassing a potential copper(II) binding site. The complexation properties of these shorter peptides were also comparatively investigated by using the same experimental approach. (literal)
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