The interaction of a peptide with a scrambled hydrophobic/hydrophilic sequence (Pro-Asp-Ala-Asp-Ala-His-Ala-His-Ala-His-Ala-Ala-Ala-His-Gly) (PADH) with DPPC model membranes: a DSC study (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• The interaction of a peptide with a scrambled hydrophobic/hydrophilic sequence (Pro-Asp-Ala-Asp-Ala-His-Ala-His-Ala-His-Ala-Ala-Ala-His-Gly) (PADH) with DPPC model membranes: a DSC study (Articolo in rivista) (literal)

Anno

• 2002-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/S0040-6031(02)00074-6 (literal)

Alternative label

• Grasso D., Milardi D., La Rosa C., Impellizzeri G, Pappalardo G.. (2002)
  The interaction of a peptide with a scrambled hydrophobic/hydrophilic sequence (Pro-Asp-Ala-Asp-Ala-His-Ala-His-Ala-His-Ala-Ala-Ala-His-Gly) (PADH) with DPPC model membranes: a DSC study
  in Thermochimica Acta
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Grasso D., Milardi D., La Rosa C., Impellizzeri G, Pappalardo G.. (literal)

Pagina inizio

• 73 (literal)

Pagina fine

• 78 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 390 (literal)

Rivista

• Thermochimica Acta (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Grasso D., La Rosa C., Impellizzeri G., Dipartimento di Scienze Chimiche, Università di Catania, Italy. Milardi D., Pappalardo G., ???? ?Istituto per lo studio delle sostanze naturali di interesse alimentare e chmico farmaceutico, Catania, CNR. ?? ? ? ff? ?? ?? ? ff? ?? ? ????? ??? ? ????????? ff? ???? ??? ????? ? ff??? ? ??? ?? ???? ??? ??? ? ff? ?? ? ????? ??? ? ????????? ff? ???? ??? ????? ? ff??? ? ??? ?? ???? ??? ??? Milardi D., Pappalardo G. (literal)

Titolo

• The interaction of a peptide with a scrambled hydrophobic/hydrophilic sequence (Pro-Asp-Ala-Asp-Ala-His-Ala-His-Ala-His-Ala-Ala-Ala-His-Gly) (PADH) with DPPC model membranes: a DSC study (literal)

Abstract

• Depending on their hydrophobicity, peptides can interact differently with lipid membranes inducing dramatic modifications into their host systems. In the present paper, the interaction of a synthetic peptide with a scrambled hydrophobic/hydrophilic sequence (Pro-Asp-Ala-Asp-Ala-His-Ala- His-Ala-His-Ala-Ala-Ala-His-Gly) (PADH) with 1,2-dipalmitoyl-sn-glycero- 3phosphocholine (DPPC) model membranes has been investigated by differential scanning calorimetry (DSC), adopting three different experimental approaches. In the first, the peptide is forced to be included into the hydrocarbon region of the lipid bilayer, by codissolving it with the lipid giving rise to mixed multilamellar vesicles-peptide systems; in the second, this system is passed through an extruder, thus producing large unilamellar vesicles-peptide systems; in the third, it is allowed to interact with the external surface of the membrane. The whole of the DSC results obtained have shown that the incorporation of the peptide into the lipid bilayer by means of the first method induces a decrease in the enthalpy of the gel-liquid crystal transition of the membrane and a shift of the transition to the lower temperatures, thus resembling, in spite of its prevalently hydrophilic nature, the behavior of transbilayer hydrophobic peptides. The extrusion of these systems creates unilamellar vesicles free of peptides but of smaller size as evidenced by the decreased cooperativity of the transition. The peptide, added externally to the DPPC model membrane, has no effect on the phase behavior of the bilayer. These findings suggest that the effect of the interaction of scrambled hydrophobic/hydrophilic peptides into lipid bilayers strongly affects the thermotropic behavior of the host membrane depending on the preparation method of the lipid/peptide systems. The whole of the results obtained in the present paper can be useful in approaching studies of bioactive peptides/lipids systems. © 2002 Elsevier Science B.V. All rights reserved. (literal)

Prodotto di

• Institute of biostructure and bioimaging (IBB) (Istituto)

Autore CNR

• DANILO MILARDI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "The interaction of a peptide with a scrambled hydrophobic/hydrophilic sequence (Pro-Asp-Ala-Asp-Ala-His-Ala-His-Ala-His-Ala-Ala-Ala-His-Gly) (PADH) with DPPC model membranes: a DSC study" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• DANILO MILARDI (Unità di personale interno)

Prodotto

• Institute of biostructure and bioimaging (IBB) (Istituto)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Thermochimica Acta (Rivista)

Insieme di parole chiave di

• Keywords of "The interaction of a peptide with a scrambled hydrophobic/hydrophilic sequence (Pro-Asp-Ala-Asp-Ala-His-Ala-His-Ala-His-Ala-Ala-Ala-His-Gly) (PADH) with DPPC model membranes: a DSC study" (Insieme di parole chiave)