Interaction of antigenic phytoplasma membrane proteins with leafhopper vector proteins: a role in transmission specificity? (Abstract/Poster in atti di convegno)

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  • Interaction of antigenic phytoplasma membrane proteins with leafhopper vector proteins: a role in transmission specificity? (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Alternative label
  • GALETTO L. , BOSCO D. 2, & MARZACHì C. (2011)
    Interaction of antigenic phytoplasma membrane proteins with leafhopper vector proteins: a role in transmission specificity?
    in EMBO Meeting 2011, Vienna, Austria
    (literal)
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  • GALETTO L. , BOSCO D. 2, & MARZACHì C. (literal)
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  • Background: Phytoplasmas are wall-less plant bacteria associated with crop diseases worldwide. They live in the plant phloem and are transmitted from plant to plant by insect vectors in a persistent and propagative manner. Since thousands of insect species feed in the phloem, but less than 100 are known phytoplasma vectors, there must be a recognition between pathogen and vectors. We identified vector proteins specifically interacting with the most abundant antigenic membrane protein (Amp) of phytoplasmas. Observations: Non-denaturing and denaturing far Western blotting and affinity chromatography showed interactions of 'Candidatus Phytoplasma asteris, CY strain Amp with several membrane proteins of the vector, among which the alpha and beta subunits of ATP synthase, and actin. No interactions with membrane proteins of non-vector insects were observed. The presence of ATP synthase, generally known as a mitochondrial protein, on the plasma membranes of the midgut and salivary gland cells of the insect vector was demonstrated by serology, immunolabelling and confocal microscopy. Membrane localization and in vitro interaction with phytoplasma membrane proteins indicated that, besides being involved in energy metabolism, ATP synthase (a multi-function protein) may act as a receptor for Amp. Amp also interacted with actin of vector, but not non-vector, insect species, consistent with previous findings for the closely related OY phytoplasma strain of 'Ca. P. asteris' and several of its vector species. Other vector proteins also interacted with Amp, but peptide mass fingerprinting could not identify them. Conclusions: Amp of CY specifically interacted with plasma membrane proteins from vector species only, among which actin and ATP synthase. Other vector proteins interacted with Amp, suggesting the presence of a complex network of insect proteins interacting with Amp in the determination of vector specificity. (literal)
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  • 2 Università di Torino, Facoltà di Agraria, Di.Va.P.R.A. Settore Entomologia e Zoologia applicate all'Ambiente \"Carlo Vidano\" Via Leonardo da Vinci, 44, 10095 Grugliasco (To) (literal)
Titolo
  • Interaction of antigenic phytoplasma membrane proteins with leafhopper vector proteins: a role in transmission specificity? (literal)
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