Peach latent mosaic viroid: further dissection of the molecular determinant inducing peach calico disease. (Comunicazione a convegno)

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  • Peach latent mosaic viroid: further dissection of the molecular determinant inducing peach calico disease. (Comunicazione a convegno) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Alternative label
  • Navarro B., Delgado S., Rodio M.E., Flores R. and Di Serio F. (2009)
    Peach latent mosaic viroid: further dissection of the molecular determinant inducing peach calico disease.
    in 21st International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Neustadt (Germany)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Navarro B., Delgado S., Rodio M.E., Flores R. and Di Serio F. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Peach calico (PC) disease is a severe chlorosis induced by Peach latent mosaic viroid (PLMVd). This symptomatology is exclusively elicited by PLMVd variants containing a specific insertion of 12-13 nt that, in the proposed secondary structure of the viroid RNA, folds into a hairpin composed by a short stem of four base pairs capped by a U-rich loop. In previous works, we showed that both the loop and the stem of this structural element play critical pathogenic roles. Here we have further dissected this structural element by site-directed mutagenesis, bioassay of the mutated variants and molecular analysis of the progenies, confirming that not only the size but also nucleotide composition of the stem strongly affect the pathogenic properties. We have also observed an uneven distribution of PLMVd variants in the chlorotic and adjacent green leaf sectors of GF-305 seedlings inoculated with in vitro dimeric transcripts of PC-inducing variants: new variants, most likely non-pathogenic and with a modified stem of five base pair capped by a U-rich loop, were generated during the infection and accumulated preferentially in the green sectors. Our data support the use of PLMVd as model system for studying the pathogenesis and evolution of non-coding RNAs. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Instituto de Biologia Molecular y Celular de Plantas (UPV-CSIC),Valencia, Spain. (literal)
Titolo
  • Peach latent mosaic viroid: further dissection of the molecular determinant inducing peach calico disease. (literal)
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