Amp-mediated interactions between “Candidatus Phytoplasma asteris” chrysanthemum yellows (CY) and leafhopper vectors (Comunicazione a convegno)

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  • Amp-mediated interactions between “Candidatus Phytoplasma asteris” chrysanthemum yellows (CY) and leafhopper vectors (Comunicazione a convegno) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Alternative label
  • Galetto L., Bosco D., Marzachì C. (2008)
    Amp-mediated interactions between “Candidatus Phytoplasma asteris” chrysanthemum yellows (CY) and leafhopper vectors
    in International Congresso of Plant Pathology, Torino
    (literal)
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  • Galetto L., Bosco D., Marzachì C. (literal)
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  • 461 (literal)
Pagina fine
  • 461 (literal)
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  • 90 (literal)
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  • 1 (literal)
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  • S2 (literal)
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  • The chrysanthemum yellows strain of the “Candidatus Phytoplasma asteris” (CY) is vectored with different efficiencies by the leafhoppers Macrosteles quadripunctulatus and Euscelidius variegatus. The molecular basis of the interactions between the phytoplasma and the vector are mainly unknown, although phytoplasma membrane proteins must be involved. The recent sequencing of few phytoplasma genomes has shown that several proteins may be predicted to have a membrane location and, in some cases, hydrophilic portions protruding from the membrane towards the extracellular environment. Among these, the CY antigenic membrane protein (Amp) and the periplasmic binding protein of the ABC-type arginine transporter system (ArtI) were chosen for further studies. In the absence of a cell wall, these proteins represent good targets to address in the search of the molecular basis of the interaction between the phytoplasma and its host. Preliminary results have shown that several vector-proteins interact specifically with CY-Amp in far-western experiments. Under non-denaturing in vitro conditions, CY Amp specifically interacted with 3 major vector proteins and, to a minor extent, with actin. Identification of the interacting vector peptides was obtained through de novo sequencing and Blast analysis. Molecular characterization of the vector genes is in progress. The pattern of vector membrane-peptides interacting with Amp and ArtI are currently under investigation. Under the same experimental conditions, the interaction of ArtI with vector proteins was aspecific. The identification of vector proteins interacting with phytoplasma membrane proteins will provide clues on the molecular mechanism regulating phytoplasma colonization of the vector body and, possibly, vector specificity. (literal)
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  • Bosco D.: Universita’ degli Studi di Torino, Di.Va.P.R.A. Torino, Italy (literal)
Titolo
  • Amp-mediated interactions between “Candidatus Phytoplasma asteris” chrysanthemum yellows (CY) and leafhopper vectors (literal)
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