http://www.cnr.it/ontology/cnr/individuo/prodotto/ID130789
Influence of HC-Pro- and p19-mediated silencing suppression on the infection of Tomato yellow leaf curl Sardinia virus (TYLCSV) (Abstract/Poster in atti di convegno)
- Type
- Label
- Influence of HC-Pro- and p19-mediated silencing suppression on the infection of Tomato yellow leaf curl Sardinia virus (TYLCSV) (Abstract/Poster in atti di convegno) (literal)
- Anno
- 2007-01-01T00:00:00+01:00 (literal)
- Alternative label
SARDO, L., WEGE, C., ACCOTTO, G.P., NORIS, E. (2007)
Influence of HC-Pro- and p19-mediated silencing suppression on the infection of Tomato yellow leaf curl Sardinia virus (TYLCSV)
in XIII International Congress on Molecular Plant-Microbe Interactions Congress, Sorrento, July 21-27, 2007
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- SARDO, L., WEGE, C., ACCOTTO, G.P., NORIS, E. (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
- Note
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- SL, AGP, NE: IVV CNR Str delle Cacce 73, 10135 Turin, Italy;
CW: Abt. Molekularbiologie und Virologie der Pflanzen, Biologisches Institut, Universität Stuttgart, Stuttgart, Germany (literal)
- Titolo
- Influence of HC-Pro- and p19-mediated silencing suppression on the infection of Tomato yellow leaf curl Sardinia virus (TYLCSV) (literal)
- Abstract
- The monopartite ssDNA begomovirus Tomato yellow leaf curl Sardinia virus (TYLCSV) is a phloem-limited pathogen of tomato. A few lines of evidence support the idea that TYLCSV might evade the gene silencing defence mechanism exerted by plants against pathogens. Besides, it has been shown that mesophyll-invasive RNA viruses harbouring strong silencing suppressors allow Abutilon mosaic virus (another phloem-limited begomovirus) to accumulate at higher titres and to infiltrate in tissues surrounding the phloem. We have undertaken a study to analyse if TYLCSV infection and tissue tropism are altered under conditions of enhanced suppression of host gene silencing and consequently of reduced host defence capacities in Nicotiana benthamiana. To this aim, two strategies have been followed. First, TYLCSV titres and tissue localisation have been tested in transgenic plants expressing two potent silencing suppressors, HC-Pro from Cowpea aphid-borne mosaic virus (CABMV, family Potyviridae) and p19 from Artichoke mottle crinkle virus (AMCV, family Tombusviridae). In a second approach, TYLCSV has been analysed in mixed infections with either CABMV or AMCV. Southern blot analysis revealed that no differential virus accumulation occurred in HC-Pro- and p19-transgenic plants compared to control wild-type plants; in situ hybridizations showed that phloem-limitation was not altered either. In mixed infections preliminary results indicate that the amount of TYLCSV is unaltered, as compared with singly infected control plants. Since HC-Pro- and p19-mediated silencing suppression does not seem to enhance TYLCSV accumulation and modify cell-to-cell spread, it is possible that TYLCSV evades gene silencing through a still unveiled mechanism, which is only acting inside the phloem domain. It remains to be determined if TYLCSV might colonise mesophyll tissues in the presence of yet unidentified factors, or if a multiplicity of adaptations confines it to the phloem permanently. (literal)
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