Cryopreservation of ancient apple cultivars of Veneto: a comparison between PVS2-vitrification and dormant-bud techniques (Comunicazione a convegno)

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  • Cryopreservation of ancient apple cultivars of Veneto: a comparison between PVS2-vitrification and dormant-bud techniques (Comunicazione a convegno) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Alternative label
  • Lambardi M., Previati A., Benelli C., Da Re F., De Carlo A., Ellis D. (2009)
    Cryopreservation of ancient apple cultivars of Veneto: a comparison between PVS2-vitrification and dormant-bud techniques
    in 1st International Symposium on Cryopreservation in Horticultural Species, Leuven, Belgio
    (literal)
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  • Lambardi M., Previati A., Benelli C., Da Re F., De Carlo A., Ellis D. (literal)
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  • Crioconservazione di gemme dormienti di antiche varietà di melo del veneto. (literal)
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  • Although the cryopreservation of vegetatively-propagated fruit species has mostly used PVS2-based vitrification procedures of shoot tips, the conservation in liquid nitrogen of dormant buds, followed by chip budding, has also been very successful for specific applications. This study has focused on the assessment of PVS2-vitrification and dormant-bud techniques on ancient apple cultivars, recovered in Veneto (North-East of Italy) and preserved in field by the Veneto Agricoltura regional agency. For PVS2-vitrification, after cold-hardening of stock cultures at 4°C for 3 weeks, shoot tips were excised and cold-hardened at 4°C for additional 2 days. The shoot tips were then exposed to a cryoprotective solution (2 M glycerol and 0.4 M sucrose) for 30 min at 25°C, treated with PVS2 for 60 or 90 min at 0°C and then directly immersed in liquid nitrogen. After thawing, the shoot tips were plated on semi-solid media, containing various combinations of growth regulators and activated charcoal. The best results of three ancient cultivars (‘Campanin’, ‘Rosetta Mantovana’ and ‘Pom dell’Oio’) ranged from 57% to 100% of shoot-tip survival after cryopreservation. The dormant-bud protocol was based on winter collection of scions from which uni-nodal sections were cut, desiccated, slow cooled (-1°C/h) up to -30°C, stored in liquid nitrogen for a minimum of 48 hours, thawed, re-hydrated and chip budded onto clonal rootstocks in a greenhouse. Of the three cultivars tested (‘San Piero’, ‘Canada Ruden’ and ‘Rosa Gentile’), best result was achieved with the cv San Piero, for which 79% of the buds survived ultra-freezing and 100% of the grafted plants regrew successfully when 26%-desiccated and cryopreserved buds were used for chip budding. A comparison between the two techniques was made based on resources required for the introduction and recover from liquid nitrogen of a single apple accession. (literal)
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  • Previati A., Da Re F.: Centro Sperimentale Ortofloricolo “Po di Tramontana”, Veneto Agricoltura, 45010 Rosolina (Rovigo), Italy. Ellis D.: National Center for Genetic Resources Preservation USDA-ARS, Fort Collins, CO 80521, USA. (literal)
Titolo
  • Cryopreservation of ancient apple cultivars of Veneto: a comparison between PVS2-vitrification and dormant-bud techniques (literal)
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