The role of Metal ions in the fibrillogenesis of beta-amyloid (Abstract/Poster in atti di convegno)

Type
Label
  • The role of Metal ions in the fibrillogenesis of beta-amyloid (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Alternative label
  • Drago D, Bettella M, Cendron L, Tognon G, Zatta P (2007)
    The role of Metal ions in the fibrillogenesis of beta-amyloid
    in III Meeting on the molecular mechanism of neurodegeneration 2007, Milano
    (literal)
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  • Drago D, Bettella M, Cendron L, Tognon G, Zatta P (literal)
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  • Metal ions are widely recognized as a key factor for conformational changes and aggregation of Alzheimer’s disease amyloid (Abeta). The purpose of this study is to compare the effects of Abeta and Abeta-metal complexes (Al, Zn, Cu, Fe) in human SHSY5Y neuroblastoma cells in terms of cell viability (MTT assay), membrane structure properties (fluorescence anisotropy), cellular and mitochondrial morphology (TEM, confocal microscopy). No significant toxic effects are observed in neuroblastoma cells after 24h treatment with Abeta and Abeta-metals (Zn, Cu, Fe), except for a significant reduction of cellular viability after treatment with Abeta-Al complex. The effects of Abeta-Al as well as other Abeta-metal complexes are also evaluated in terms of fluorescence anisotropy in order to consider the possible alteration in the membrane structure properties. In this connection, treatment with Abeta-Al is able to increase specifically the membrane fluidity with respect to other Abeta-metal complexes. Moreover, the toxic role of Abeta-Al is also correlated with a significant alteration in cellular morphology shown by electron microscopy (TEM). Mitochondrial functionality could be compromised after treatment with Abeta-Al complex as reported in confocal micrographs of neuroblatoma cells stained with Mitotracker Red. (literal)
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  • Istitute for Biomedical Technologies-CNR-Padua Section (literal)
Titolo
  • The role of Metal ions in the fibrillogenesis of beta-amyloid (literal)
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