Analysis by phage display selection and site-directed retromutagenesis of the Mustard Trypsin Inhibitor 2 reactive site (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Analysis by phage display selection and site-directed retromutagenesis of the Mustard Trypsin Inhibitor 2 reactive site (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Alternative label

• Volpicella M; Leoni C; Arnesano F; Gallerani R; Ceci LR (2010)
  Analysis by phage display selection and site-directed retromutagenesis of the Mustard Trypsin Inhibitor 2 reactive site
  in Journal of plant physiology (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Volpicella M; Leoni C; Arnesano F; Gallerani R; Ceci LR (literal)

Pagina inizio

• 1507 (literal)

Pagina fine

• 1511 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 167 (literal)

Rivista

• Journal of plant physiology (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Biomembrane e Bioenergetica (literal)

Titolo

• Analysis by phage display selection and site-directed retromutagenesis of the Mustard Trypsin Inhibitor 2 reactive site (literal)

Abstract

• The Mustard Trypsin Inhibitor (MSI) family is a small family of plant protease inhibitors so far only found in Brassicaceae. Using a phage display selection, MTI-2 (Mustard Trypsin Inhibitor 2) mutants were detected and analysed for their biochemical characteristics. Retromutants of the selected MTI-2 proteins were constructed and expressed in the Pichia pastoris system. The recombinant proteins were analysed by activity assays against bovine trypsin and Helicoverpa zea trypsin, and by circular dichroism. These analyses suggest a strict requirement for a specific proline residue adjacent to the inhibitor reactive site and give additional insights for future phage display application. (literal)

Prodotto di

• Institute of biomembrane and bioenergetics (IBBE) (Istituto)
• Biogenesi delle Membrane di Trasduzione dell'Energia. (SV.P13.002.001) (Modulo)

Autore CNR

• LUIGI RUGGIERO CECI (Persona)

Incoming links:

Prodotto

• Institute of biomembrane and bioenergetics (IBBE) (Istituto)
• Biogenesi delle Membrane di Trasduzione dell'Energia. (SV.P13.002.001) (Modulo)

Autore CNR di

• LUIGI RUGGIERO CECI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of plant physiology (Rivista)