http://www.cnr.it/ontology/cnr/individuo/prodotto/ID12877

Soluble proteome investigation of cobalt effect on the carotenoidless mutant of Rhodobacter sphaeroides. (Articolo in rivista)

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Soluble proteome investigation of cobalt effect on the carotenoidless mutant of Rhodobacter sphaeroides. (Articolo in rivista) (literal)

Anno

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Alternative label

Pisani F, Italiano F, de Leo F, Gallerani R, Rinalducci S, Zolla L, Agostiano A, Ceci LR, Trotta M. (2009)
Soluble proteome investigation of cobalt effect on the carotenoidless mutant of Rhodobacter sphaeroides.
in Journal of applied microbiology (Print)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Pisani F, Italiano F, de Leo F, Gallerani R, Rinalducci S, Zolla L, Agostiano A, Ceci LR, Trotta M. (literal)

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1. CNR, Ist Proc Chim Fis, I-70126 Bari, Italy 2. Univ Bari, Dipartmento Biochim & Biol Mol, Bari, Italy 3. Univ Tuscia, Dipartimento Sci Ambientali, Viterbo, Italy 4. Univ Bari, Dipartimento Chim, Bari, Italy 5. CNR, Ist Biomembrane & Bioenerget, Trani, Italy 6. CNR, Ist Biomembrane & Bioenerget, Bari, Italy (literal)

Titolo

Soluble proteome investigation of cobalt effect on the carotenoidless mutant of Rhodobacter sphaeroides. (literal)

Abstract

To investigate the surviving capability of Rhodobacter sphaeroides under phototrophic conditions in the presence of high cobalt concentration and its influence on the photosynthetic apparatus biosynthesis. Cells from R. sphaeroides strain R26.1 were grown anaerobically in a medium containing 5.0 mmol l(-1) cobalt ions and in a control medium. Metal toxicity was investigated comparing the soluble proteome of Co(2+)-exposed cells and cells grown in control medium by two-dimensional gel electrophoretic analysis. Significant changes in the expression level were detected for 43 proteins, the majority (35) being up-regulated. The enzyme porphobilinogen deaminase (PBGD) was found down-regulated and its activity was investigated. The up-regulated enzymes mainly belong to the general category of proteins and DNA degradation enzymes, suggesting that part of the catabolic reaction products can rescue bacterial growth in photosynthetically impaired cells. Furthermore, the down-regulation of PBGD strongly indicates that this key enzyme of the tetrapyrrole and bacteriochlorophyll synthesis is directly involved in the metabolic response. Data and experiments show that the cobalt detrimental effect on the photosynthetic growth of R. sphaeroides is associated with an impaired expression and functioning of PBGD. (literal)

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