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Accumulation of overoxidized Peroxiredoxin III in aged rat liver mitochondria (Articolo in rivista)

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Accumulation of overoxidized Peroxiredoxin III in aged rat liver mitochondria (Articolo in rivista) (literal)

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Musicco C (a); Capelli V (b); Pesce V (b); Timperio AM (c); Calvani M (d); Mosconi L (d); Zolla L (c); Cantatore P (a,b); Gadaleta MN (a,b). (2009)
Accumulation of overoxidized Peroxiredoxin III in aged rat liver mitochondria
in Biochimica et biophysica acta. Bioenergetics; Elsevier, Amsterdam (Paesi Bassi)
(literal)

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Musicco C (a); Capelli V (b); Pesce V (b); Timperio AM (c); Calvani M (d); Mosconi L (d); Zolla L (c); Cantatore P (a,b); Gadaleta MN (a,b). (literal)

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a)Institute of Biomembranes and Bioenergetics, Consiglio Nazionale delle Ricerche (CNR), Bari, Italy b)Department of Biochemistry and Molecular Biology \"Ernesto Quagliariello\", University of Bari, Bari, Italy c)Department of Environmental Sciences, Tuscia University, Viterbo, Italy d)Sigma Tau - Industrie Farmaceutiche Riunite S.p.A., Pomezia (Roma), Italy (literal)

Titolo

Accumulation of overoxidized Peroxiredoxin III in aged rat liver mitochondria (literal)

Abstract

Overoxidation and subsequent inactivation of Peroxiredoxin III (PrxIII), a mitochondrial H2O2 scavenging enzyme, have been reported in oxidative stress conditions. No data are available in the literature about the presence of overoxidized forms of PrxIII in aged tissues. Liver mitochondria from 12-month-old rats and 28-month-old rats were here analyzed by two-dimensional gel electrophoresis. A spot corresponding to the native form of PrxIII was present in adult and old rats with the same volume, whereas an additional, more acidic spot, of the same molecular weight of the native form, accumulated only in old rats. The acidic spot was identified, by MALDI-MS analysis, as a form of PrxIII bearing the cysteine of the catalytic site overoxidized to sulphonic acid. This modified PrxIII form corresponds to the irreversibly inactivated enzyme, here reported, for the first time, in aging. Three groups of 28-month-old rats treated with acetyl-L-carnitine were also examined. Reduced accumulation of the overoxidized PrxIII form was found in all ALCAR-treated groups. (literal)

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