Loss of BRCA2 promotes prostate cancer cell invasion through up-regulation of matrix metalloproteinase-9. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Loss of BRCA2 promotes prostate cancer cell invasion through up-regulation of matrix metalloproteinase-9. (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Alternative label

• Moro L, Arbini AA, Yao JL, di Sant'Agnese PA, Marra E, Greco M. (2008)
  Loss of BRCA2 promotes prostate cancer cell invasion through up-regulation of matrix metalloproteinase-9.
  in Cancer science
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Moro L, Arbini AA, Yao JL, di Sant'Agnese PA, Marra E, Greco M. (literal)

Pagina inizio

• 553 (literal)

Pagina fine

• 563 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 99 (literal)

Rivista

• Cancer science (Rivista)

Note

• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CNR, Istituto di Biomembrane e Bioenergetica, Bari Department of Pathology, UT South-western Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 Department of Pathology and Laboratory Medicine, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA (literal)

Titolo

• Loss of BRCA2 promotes prostate cancer cell invasion through up-regulation of matrix metalloproteinase-9. (literal)

Abstract

• BRCA2 is a multifunctional tumor suppressor protein which plays critical roles in DNA repair, transcription, and cell proliferation, and the loss of which has been linked to the biology of several types of cancers. Here, on prostate adenocarcinoma specimens from 80 patients, we demonstrate that BRCA2 protein is lost in carcinoma cells compared to normal and hyperplastic prostate epithelium. Using highly metastatic prostate cancer PC-3 cells, we show that while BRCA2 depletion by small-interfering RNA promoted migration onto the extracellular matrix proteins fibronectin, laminin, and collagens, as well as invasion through the reconstituted basement membrane matrix Matrigel by more than 140%, recombinant BRCA2 overexpression decreased both phenomena by 57-80% and changed cell morphology from angular and spindle to round and compact. The BRCA2 inhibitory effect on cancer cell migration and invasion resulted from down-regulation of matrix metalloproteinase (MMP)-9 protein levels due to increased MMP-9 proteolysis, and was signaled through inhibition of PI3-kinase/AKT and activation of MAPK/ERK pathway. In BRCA2-overexpressing PC-3 cells, transient transfection with a constitutively act (literal)

Prodotto di

• Istituto di biomembrane e bioenergetica (IBBE) (Istituto)
• Interrelazione nucleo/citoplasma/mitocondri nell'omeostasi cellulare. (SV.P15.003.001) (Modulo)

Autore CNR

• LOREDANA MORO (Unità di personale interno)
• ERSILIA MARRA (Unità di personale interno)
• MARGHERITA GRECO (Persona)

Insieme di parole chiave

• Keywords of "Loss of BRCA2 promotes prostate cancer cell invasion through up-regulation of matrix metalloproteinase-9." (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di biomembrane e bioenergetica (IBBE) (Istituto)
• Interrelazione nucleo/citoplasma/mitocondri nell'omeostasi cellulare. (SV.P15.003.001) (Modulo)

Autore CNR di

• LOREDANA MORO (Unità di personale interno)
• MARGHERITA GRECO (Persona)
• ERSILIA MARRA (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Cancer science (Rivista)

Insieme di parole chiave di

• Keywords of "Loss of BRCA2 promotes prostate cancer cell invasion through up-regulation of matrix metalloproteinase-9." (Insieme di parole chiave)