Functional reconstitution into liposomes and characterization of the carnitine transporter from rat liver microsomes (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Functional reconstitution into liposomes and characterization of the carnitine transporter from rat liver microsomes (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.bbamem.2006.01.004 (literal)

Alternative label

• Annamaria Tonazzi , Michele Galluccio , Francesca Oppedisano , Cesare Indiveri (2006)
  Functional reconstitution into liposomes and characterization of the carnitine transporter from rat liver microsomes
  in Biochimica et biophysica acta. Bioenergetics
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Annamaria Tonazzi , Michele Galluccio , Francesca Oppedisano , Cesare Indiveri (literal)

Pagina inizio

• 124 (literal)

Pagina fine

• 131 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 1758 (literal)

Rivista

• Biochimica et biophysica acta. Bioenergetics (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• National Research Council Institute of Biomembranes and Bioenergetics (IBBE), via Amendola 165/A, 70126, Bari, Italy Department of Cellular Biology, University of Calabria, Via P.Bucci cubo 4c, 87036 Arcavacata di Rende (CS), Italy (literal)

Titolo

• Functional reconstitution into liposomes and characterization of the carnitine transporter from rat liver microsomes (literal)

Abstract

• The carnitine transporter was solubilized from rat liver microsomes with Triton X-100 and reconstituted into liposomes, after addition of Triton X- 114, by removing the detergent from mixed micelles by hydrophobic chromatography on Amberlite (Bio-Beads SM 2). The reconstitution was optimized with respect to the detergent/phospholipid ratio, the protein concentration, and the number of passages through a single Amberlite column. The reconstituted carnitine transporter catalyzed a first-order uniport reaction inhibited by HgCl2 and DIDS. The IC50 for HgCl2 was 0.16±0.03 mM. The reconstituted transporter also catalyzed carnitine efflux from the proteoliposomes; the efflux was stimulated by externally added long-chain acylcarnitines. Besides carnitine, ornithine, arginine, glutamine and lysine were taken up by the reconstituted liposomes with lower efficiency respect to carnitine. Optimal activity was found at pH 8.0. The Km for carnitine on the external side of the transporter was 10.9±0.16 mM. The activation energy of the carnitine transport derived by Arrhenius plot was 16.1 kJ/mol. (literal)

Prodotto di

• Trasportatori mitocondriali: struttura e meccanismi funzionali (SV.P14.007.001) (Modulo)
• Istituto di biomembrane e bioenergetica (IBBE) (Istituto)

Autore CNR

• ANNAMARIA TONAZZI (Persona)

Insieme di parole chiave

• Parole chiave di "Functional reconstitution into liposomes and characterization of the carnitine transporter from rat liver microsomes" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di biomembrane e bioenergetica (IBBE) (Istituto)
• Trasportatori mitocondriali: struttura e meccanismi funzionali (SV.P14.007.001) (Modulo)

Autore CNR di

• ANNAMARIA TONAZZI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Biochimica et biophysica acta. Bioenergetics (Rivista)

Insieme di parole chiave di

• Parole chiave di "Functional reconstitution into liposomes and characterization of the carnitine transporter from rat liver microsomes" (Insieme di parole chiave)