Simultaneous determination of purine nucleotides, their metabolites and b-nicotinamide adenine inucleotide in cerebellar granule cells by ion-pair high performance liquid chromatography. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Simultaneous determination of purine nucleotides, their metabolites and b-nicotinamide adenine inucleotide in cerebellar granule cells by ion-pair high performance liquid chromatography. (Articolo in rivista) (literal)

Anno

• 2003-01-01T00:00:00+01:00 (literal)

Alternative label

• Giannattasio S., Gagliardi S., Samaja M., Marra E. (2003)
  Simultaneous determination of purine nucleotides, their metabolites and b-nicotinamide adenine inucleotide in cerebellar granule cells by ion-pair high performance liquid chromatography.
  in Brain research protocols
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Giannattasio S., Gagliardi S., Samaja M., Marra E. (literal)

Pagina inizio

• 168 (literal)

Pagina fine

• 174 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 10 (literal)

Rivista

• Brain research protocols (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CNR - Istituto di Biomembarne e Bioenergetica, Bari Dipartimento di Medicina, Chirurgia e Odontoiatria, Universita` di Milano, Milano, Italy (literal)

Titolo

• Simultaneous determination of purine nucleotides, their metabolites and b-nicotinamide adenine inucleotide in cerebellar granule cells by ion-pair high performance liquid chromatography. (literal)

Abstract

• The method described here allows the quantitative simultaneous determination of adenosine 5´-triphosphate, adenosine 5´-diphosphate, adenosine 5´-monophosphate, adenosine, guanosine 5´-triphosphate, guanosine 5´-diphosphate, guanosine, inosine 5´-monophosphate, inosine, uric acid, xanthine, hypoxanthine and b-nicotinamide adenine dinucleotide by ion-pair high performance liquid chromatography. The chromatographic analysis requires 26 min per sample and allows the separation of the mentioned metabolites in a time as short as 16 min. Primary cultures of rat cerebellar granule cells were incubated in serum-free medium containing 25 mM KCl for 1.5–48 h and their acid extracts were injected onto column. Uric acid, inosine 5´-monophosphate, inosine, b-nicotinamide adenine dinucleotide, adenosine, adenosine 5´-monophosphate, guanosine 5´-diphosphate, adenosine 5´-diphosphate, guanosine 5´-triphosphate and adenosine 5´-triphosphate were identified and quantified, while hypoxanthine, xanthine and guanosine were below the detection limit. This method makes use of a single-step sample pre-treatment procedure which allows a greater than 91% recovery of the compounds of interest and provides the assay of the metabolites of interest in little amounts of cell extracts. Therefore, this method is suitable to evaluate the energetic state in a variety of cell types, both under normal and dismetabolic conditions, such as after the induction of apoptosis or necrosis. (literal)

Prodotto di

• Institute of biomembrane and bioenergetics (IBBE) (Istituto)

Autore CNR

• SERGIO GIANNATTASIO (Unità di personale interno)
• ERSILIA MARRA (Persona)

Incoming links:

Prodotto

• Institute of biomembrane and bioenergetics (IBBE) (Istituto)

Autore CNR di

• SERGIO GIANNATTASIO (Unità di personale interno)
• ERSILIA MARRA (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Brain research protocols (Rivista)