Stress response gene activation protects sea urchin embryos exposed to X-rays. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Stress response gene activation protects sea urchin embryos exposed to X-rays. (Articolo in rivista) (literal)

Anno

• 2011-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/s12192-011-0277-3 (literal)

Alternative label

• Bonaventura R; Zito F; Costa C; Giarrusso S; Celi F; Matranga V. (2011)
  Stress response gene activation protects sea urchin embryos exposed to X-rays.
  in Cell stress & chaperones
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Bonaventura R; Zito F; Costa C; Giarrusso S; Celi F; Matranga V. (literal)

Pagina inizio

• 681 (literal)

Pagina fine

• 687 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 16 (literal)

Rivista

• Cell stress & chaperones (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• * IN PRESS (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• - Consiglio Nazionale delle Ricerche, Istituto di Biomedicina e Immunologia Molecolare \"Alberto Monroy\", Palermo, Italy - INAF, Istituto di Astrofisica Spaziale e Fisica Cosmica, Palermo, Italy (literal)

Titolo

• Stress response gene activation protects sea urchin embryos exposed to X-rays. (literal)

Abstract

• We used Paracentrotus lividus sea urchin embryos, a well-established model in developmental biology and ecotoxicology, for investigation on stress/anti-apoptotic protein expression elicited in response to harmful ionizing radiation, such as X-rays. We evaluated the acute effects of a high-dose exposure (5 Gy) on P. lividus analyzing by Western blotting the accumulation levels of HSP60, HSP70, BAG3 and a putative p63 at 24 and 48 h after irradiation. We found an increase in the HSP70, BAG3, and p63 protein levels only 48 h after irradiation, whereas no HSP60 increase was detected either at 24 or 48 h. Levels of the mRNA coding for HSP70 and p63 were also investigated by relative RT-PCR and were found to increase 24 h after irradiation, returning to their initial levels at 48 h. Results demonstrate the presence of an adaptive regulatory mechanism operating at the transcriptional level at 24 h, followed by a translational activation at 48 h postirradiation. In conclusion, our findings confirm the sea urchin embryo as a sensible bioindicator of cell damage and we propose this model for studies on the protective pathways activated in response to X-rays. The novel result of the involvement of BAG3 and p63 in the response to X-rays, never tested so far in any other embryonic system, opens the way for their use as biomarkers of X-ray hazards. (literal)

Prodotto di

• Stress Cellulare ed Ambiente (SV.P17.008.003) (Modulo)
• Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (IBIM) (Istituto)

Autore CNR

• FRANCESCA ZITO (Persona)
• VALERIA MATRANGA (Unità di personale interno)
• CATERINA COSTA (Unità di personale interno)
• ROSA BONAVENTURA (Unità di personale interno)

Incoming links:

Autore CNR di

• VALERIA MATRANGA (Unità di personale interno)
• CATERINA COSTA (Unità di personale interno)
• FRANCESCA ZITO (Persona)
• ROSA BONAVENTURA (Unità di personale interno)

Prodotto

• Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (IBIM) (Istituto)
• Stress Cellulare ed Ambiente (SV.P17.008.003) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Cell stress & chaperones (Rivista)