Leukotriene B4 production in human mononuclear phagocytes is modulated by IL-4-induced 15-lipoxygenase. (Articolo in rivista)

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Label
  • Leukotriene B4 production in human mononuclear phagocytes is modulated by IL-4-induced 15-lipoxygenase. (Articolo in rivista) (literal)
Anno
  • 2002-01-01T00:00:00+01:00 (literal)
Alternative label
  • MIRELLA PROFITA, ANGELO SALA, LIBORIA SIENA, PETER M. HENSON, ROBERT C. MURPHY, ALESSANDRA PATERNO` , ANNA BONANNO, LOREDANA RICCOBONO, ANGELA MIRABELLA, GIOVANNI BONSIGNORE, and ANTONIO M. VIGNOLA (2002)
    Leukotriene B4 production in human mononuclear phagocytes is modulated by IL-4-induced 15-lipoxygenase.
    in The journal of pharmacology and experimental therapeutics (Online)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • MIRELLA PROFITA, ANGELO SALA, LIBORIA SIENA, PETER M. HENSON, ROBERT C. MURPHY, ALESSANDRA PATERNO` , ANNA BONANNO, LOREDANA RICCOBONO, ANGELA MIRABELLA, GIOVANNI BONSIGNORE, and ANTONIO M. VIGNOLA (literal)
Pagina inizio
  • 868 (literal)
Pagina fine
  • 875 (literal)
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  • 300 (literal)
Rivista
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  • 3 (literal)
Note
  • PubMe (literal)
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  • Istituto di Fisiopatologia Respiratoria, Consiglio Nazionale delle Ricerche, Palermo, Italy (M.P., L.S., A.P., A.B., L.R., G.B.); Dipartimento di Scienze Famacologiche, Universita` di Milano, Milan, Italy (A.S.); National Jewish Medical and Research Center, Denver, Colorado (A.S., P.M.H., R.C.M.); and Istituto di Medicina Generale e Pneumologie, Universita` di Palermo, Palermo, Italy (A.M., A.M.V.). (literal)
Titolo
  • Leukotriene B4 production in human mononuclear phagocytes is modulated by IL-4-induced 15-lipoxygenase. (literal)
Abstract
  • The aim of this study was to evaluate the consequences of interleukin (IL)-4-induced 15-lipoxygenase (15-LO) expression on leukotriene B4 (LTB4) synthesis in human monocytes. Human monocytes incubated for 24, 48, and 72 h with IL-4 (10 ng/ml) were stimulated with Ca2?-ionophore A23187 (calcimycin; 5 ?M) or opsonized zymosan. 15(S)-hydroxyeicosatetraenoic acid [15(S)-HETE], LTB4, and arachidonic acid (AA) release were measured by high-performance liquid chromotography/ radioimmunoassay, liquid chromotography/tandem mass spectrometry (LC/MS/MS), or gas chromatography/mass spectrometry. 15-LO activity was evaluated in AA-treated monocytes. 15-LO, 5-lipoxygenase (5-LO) and 5-LO activating protein (FLAP) expression were analyzed by reverse transcription- polymerase chain reaction. Neutrophil chemotactic activity was evaluated using a microtaxis chamber assay. A23187- induced synthesis of 15(S)-HETE was significantly increased after treatment with IL-4 (10 ng/ml) for 48 and 72 h (p ? 0.001). Concomitant decrease of LTB4 release was observed after 72 h of incubation with IL-4 (p ? 0.001). LC/MS/MS analysis confirmed the production of 15(S)-HETE and the significant inhibition of LTB4 synthesis in IL-4-treated monocyte after challenge with opsonized zymosan. IL-4 treatment induced 15-LO enzymatic activity as well as 15-LO mRNA, but did not affect either 5-LO or FLAP mRNA expression in monocytes. Supernatant from IL-4-treated monocytes showed significantly lower neutrophil chemotactic activity than controls. 15(S)-HETE significantly inhibited LTB4 production induced by A23187-stimulated human monocytes without affecting AA release. IL-4- induced expression of 15-LO in monocytes caused a significant reduction of LTB4 production. Whereas this effect did not reflect changes in 5-LO and FLAP mRNA expression, synthetic 15(S)-HETE was able to significantly inhibit the synthesis of LTB4, without affecting AA release. (literal)
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