Proficiency test for simultaneous determination of up to eleven mycotoxins in maize by using LC-MS(MS) methodology. (Comunicazione a convegno)

Type
Label
  • Proficiency test for simultaneous determination of up to eleven mycotoxins in maize by using LC-MS(MS) methodology. (Comunicazione a convegno) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Alternative label
  • Solfrizzo M., De Girolamo A., Lattanzio V.M.T., Visconti A., Stroka J., Alldrick A.J., van Egmond H.P. (2011)
    Proficiency test for simultaneous determination of up to eleven mycotoxins in maize by using LC-MS(MS) methodology.
    in 125th AOAC annual meeting., New Orleans, Luoisiana, 18-21 Settembre 2011
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Solfrizzo M., De Girolamo A., Lattanzio V.M.T., Visconti A., Stroka J., Alldrick A.J., van Egmond H.P. (literal)
Pagina inizio
  • 86 (literal)
Pagina fine
  • 87 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#titoloVolume
  • 125th AOAC Annual Meeting and Exposition, Educate Network Collaborate (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Institute of Sciences of Food Production, National Research Council of Italy, Via Amendola, 122/O, 70126 Bari, Italy Institute for Reference Materials and Measurements (IRMM), Retieseweg 111, B-2440 Geel, Belgium Campden BRI, Chipping Campden GL55 6LD, United Kingdom Cluster Natural Toxins and Pesticides, RIKILT - Institute of Food Safety, Wageningen UR, P.O. box 230, 6700 AE Wageningen, The Netherlands (literal)
Titolo
  • Proficiency test for simultaneous determination of up to eleven mycotoxins in maize by using LC-MS(MS) methodology. (literal)
Abstract
  • A recent survey on the use and application of methods for the determination of mycotoxins in food and feed revealed that 42% of participant laboratories routinely use LC-MS(MS) methodology for their single or simultaneous determination. However, within both CEN and AOAC contexts there are no LC-MS(MS) official methods for the measurement of this group of contaminants. A proficiency test (PT) for the simultaneous determination of up to 11 mycotoxins in maize using LC-MS(MS) methodology was conducted to obtain information on currently used methodologies and method-related performances. Each participant received a mixed mycotoxins calibrant solution, a spiking solution, a spiking protocol, instructions, a comprehensive questionnaire and two test materials (a naturally contaminated maize sample and a blank maize sample to be spiked with a mixture of 11 mycotoxins (aflatoxins B1, B2, G1 and G2, ochratoxin A, deoxynivalenol, T-2 toxin, HT-2 toxin, zearalenone, fumonisins B1 and B2). Of the 64 laboratories enrolled in the PT, 41 laboratories from 14 countries returned results for various combinations of analytes. The percentage of satisfactory z-scores (below an absolute value of 2) for naturally contaminated maize ranged from 50-52% for fumonisins to 68-84% for aflatoxins; comparable results were also obtained for spiked maize. In terms of the methodologies used, the majority of laboratories (54%) used sample extract cleanup (immunoaffinity chromatography, solid phase extraction, liquid/liquid extraction), a group of 39% analyzed a crude extract without purification whereas no information were obtained from few laboratories (7%). Electrospray ionization was used by all laboratories whereas triple quadrupole and ion trap detectors were used by 88% and 12% laboratories, respectively. For quantification, the majority of laboratories (76%) prepared calibration curves in pure solvents (with or without addition of internal standards to the sample extracts) whereas the other laboratories prepared matrix-assisted calibration curves to compensate for matrix effects. (literal)
Prodotto di
Autore CNR

Incoming links:


Autore CNR di
Prodotto
data.CNR.it