Proteomic analysis of the effect of GLRAV-1 and GVA infection on “Nebbiolo” berries at maturity. (Abstract/Poster in atti di convegno)

Type
Label
  • Proteomic analysis of the effect of GLRAV-1 and GVA infection on “Nebbiolo” berries at maturity. (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Alternative label
  • Giribaldi M., Purrotti M., Pacifico D., Santini D., Cavallarin L., Giuffrida M.G., Conti A. (2010)
    Proteomic analysis of the effect of GLRAV-1 and GVA infection on “Nebbiolo” berries at maturity.
    in Meeting MACROWINE 2010: macromolecules and secondary metabolites of grapevine and wines, Torino, 16-18/06/2010
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Giribaldi M., Purrotti M., Pacifico D., Santini D., Cavallarin L., Giuffrida M.G., Conti A. (literal)
Pagina inizio
  • 60 (literal)
Pagina fine
  • 60 (literal)
Note
  • Poster (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • GM, PM, CL, GMG, CA: ISPA CNR, via L. da Vinci 44, 10095 Grugliasco (TO) - Italy PD, SD: IVV CNR, Strada delle Cacce 73, 10135 Torino - Italy. (literal)
Titolo
  • Proteomic analysis of the effect of GLRAV-1 and GVA infection on “Nebbiolo” berries at maturity. (literal)
Abstract
  • Beside the severe sanitary control required to propagate plant material, the presence of viruses in North Western Italian vineyards is rather endemic. Viral infections are known to have a detrimental effect on vine yield and performances, but there is still a lack in the knowledge of their effect on quality and safety of final products. Previous report on Grapevine Leafroll Virus 3 (GLRaV-3) effect on grape leaves indicated that the infection increased the expression of some stress related genes, of genes involved in sugar transport, of a lipid transfer protein coding transcript, known to cause allergies to grape and wine, and of a glutathione S transferase, involved in anthocyanin storage in vacuoles [1]. No data have been already reported on virus effect on berry transcripts or proteins. We used 2D electrophoresis to separate and analyze the virus-induced changes in protein expression in the Nebbiolo grape berry. Healthy and virus-infected progenies of Nebbiolo clone 308 cultivated in Neive vineyard were screened to confirm their sanitary status and analyzed for some vegetative and yield parameters and for soil characteristics. The infected clones presented a mixed infection of both GLRaV-1 and Grapevine Virus A (GVA). Ripe berries from 20 healthy and infected plants were dissected into pulp and skin. A phenol based protocol [2] was used for skin protein extraction, while a TCA/acetone based method [3] was used for pulps. Sixty micrograms of proteins were then subjected to isoelecrofocusing on a 3-10 non linear pH gradient in the first dimension and to a 8-16% SDS polyacrilamide gradient gel in the second dimension, following Laemmli procedure [4]. Gels were then stained with colloidal Coomassie brilliant blue [5] and acquired using a CCD camera. Images were then analyzed using both PDQuest (Biorad) and Samespot (Invitrogen) softwares. ANOVA analysis revealed significant differences for 13 pulp spots and 9 skin spots, and their identification is currently underway through MALDI TOF mass spectrometry. (literal)
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