Human adipose tissue binds and metabolizes the endocannabinoids anandamide and 2-arachidonoylglycerol (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Human adipose tissue binds and metabolizes the endocannabinoids anandamide and 2-arachidonoylglycerol (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Alternative label

• Spoto B, Fezza F, Parlongo G, Battista N, Sgro' E, Gasperi V, Zoccali C, Maccarrone M. (2006)
  Human adipose tissue binds and metabolizes the endocannabinoids anandamide and 2-arachidonoylglycerol
  in Biochimie (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Spoto B, Fezza F, Parlongo G, Battista N, Sgro' E, Gasperi V, Zoccali C, Maccarrone M. (literal)

Pagina inizio

• 1889 (literal)

Pagina fine

• 1897 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 88 (literal)

Rivista

• Biochimie (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CNR, Natl Res Council Inst Biomed Clin Epidemiol & Phy, IBIM, I-89125 Reggio Di Calabria, Italy Univ Roma Tor Vergata, Dept Expt Med & Biochem Sci, I-00133 Rome, Italy Mondino Tor Vergata Ctr Expt Neurophamracol, IRCCS, Neurol Inst C Mondino Fdn, Neurochem Lab, I-00133 Rome, Italy Univ Teramo, Dept Biomed Sci, I-64100 Teramo, Italy (literal)

Titolo

• Human adipose tissue binds and metabolizes the endocannabinoids anandamide and 2-arachidonoylglycerol (literal)

Abstract

• Endocannabinoids are a group of biologically active endogenous lipids that have recently emerged as important mediators in energy balance control. The two best studied endocannabinoids, anandamide (N-arachidonoylethanolamine, AEA) and 2-arachidonoylglycerol (2-AG) are the endogenous ligands of the central and peripheral cannabinoid receptors. Furthermore, AEA binds to the transient receptor potential vanilloid type-1 (TRPV1), a capsaicin-sensitive, non-selective cation channel. The synthesis of these endocannabinoids is catalyzed by the N-acylphosphatidylethanolamine-selective phospholipase D (NAPE-PLD) and the sn-1-selective diacylglycerol lipase (DAGL), whereas their degradation is accomplished by the fatty acid amide hydrolase (FAAH) and the monoglyceride lipase (MGL), respectively. We investigated the presence of a functional endocannabinoid system in human adipose tissue from seven healthy subjects. Subcutaneous abdominal adipose tissue underwent biochemical and molecular biology analyses, aimed at testing the expression of this system and its functional activity. AEA and 2-AG levels were detected and quantified by HPLC. Real time PCR analyzed the expression of the endocannabinoid system and immunofluorescence assays showed the distribution of its components in the adipose tissue. Furthermore, binding assay for the cannabinoid and vanilloid receptors and activity assay for each metabolic enzyme of the endocannabinoid system gave clear evidence of a fully operating system. The data presented herein show for the first time that the human adipose tissue is able to bind AEA and 2-AG and that it is endowed with the biochemical machinery to metabolize endocannabinoids. (c) 2006 Elsevier Masson SAS. All rights reserved. (literal)

Prodotto di

• Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (IBIM) (Istituto)
• Epidemiologia Clinica del Rischio Cardiovascolare nelle nefropatie croniche (ME.P07.001.001) (Modulo)

Autore CNR

• CARMINE ZOCCALI (Persona)
• BELINDA GILDA SPOTO (Persona)

Incoming links:

Prodotto

• Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (IBIM) (Istituto)
• Epidemiologia Clinica del Rischio Cardiovascolare nelle nefropatie croniche (ME.P07.001.001) (Modulo)

Autore CNR di

• CARMINE ZOCCALI (Persona)
• BELINDA GILDA SPOTO (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Biochimie (Rivista)