Leukotriene B4 production in human mononuclear phagocytes is modulated by interleukin-4 induced 15 lipoxygenase (Articolo in rivista)

Type
Label
  • Leukotriene B4 production in human mononuclear phagocytes is modulated by interleukin-4 induced 15 lipoxygenase (Articolo in rivista) (literal)
Anno
  • 2002-01-01T00:00:00+01:00 (literal)
Alternative label
  • Profita M, Sala A, Siena L, Henson PM, Murphy RC, Paterno A, Bonanno A, Riccobono L, Mirabella A, Bonsignore G, Vignola AM. (2002)
    Leukotriene B4 production in human mononuclear phagocytes is modulated by interleukin-4 induced 15 lipoxygenase
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Profita M, Sala A, Siena L, Henson PM, Murphy RC, Paterno A, Bonanno A, Riccobono L, Mirabella A, Bonsignore G, Vignola AM. (literal)
Pagina inizio
  • 868 (literal)
Pagina fine
  • 875 (literal)
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  • 3000 (literal)
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  • Impact Factor: 3.555 (literal)
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  • Valutazione dell'induzione dell'enzima 15-liposigenasi in monociti di sangue periferico stimolati con Interleukina. Identificazione del ruolo dell 15-lipossigenase nell'asma attraverso uno studio in vitro (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Angelo Sala :professore associato del Centro di Farmacologia Cardiopolmonare dell'Università di Milano - Vignola AM - Istituto di Medicina Generale e Pneumologia - Responsabile Sezione IFC-IBIM-CNR (literal)
Titolo
  • Leukotriene B4 production in human mononuclear phagocytes is modulated by interleukin-4 induced 15 lipoxygenase (literal)
Abstract
  • The aim of this study was to evaluate the consequences of interleukin (IL)-4-induced 15-lipoxygenase (15-LO) expression on leukotriene B4 (LTB4) synthesis in human monocytes. Human monocytes incubated for 24, 48, and 72 h with IL-4 (10 ng/ml) were stimulated with Ca2+-ionophore A23187 (calcimycin; 5 microM) or opsonized zymosan. 15(S)-hydroxyeicosatetraenoic acid [15(S)-HETE], LTB4, and arachidonic acid (AA) release were measured by high-performance liquid chromatography/radioimmunoassay, liquid chromatography/tandem mass spectrometry (LC/MS/MS), or gas chromatography/mass spectrometry. 15-LO activity was evaluated in AA-treated monocytes. 15-LO, 5-lipoxygenase (5-LO) and 5-LO activating protein (FLAP) expression were analyzed by reverse transcription-polymerase chain reaction. Neutrophil chemotactic activity was evaluated using a microtaxis chamber assay. A23187-induced synthesis of 15(S)-HETE was significantly increased after treatment with IL-4 (10 ng/ml) for 48 and 72 h (p < 0.001). Concomitant decrease of LTB4 release was observed after 72 h of incubation with IL-4 (p < 0.001). LC/MS/MS analysis confirmed the production of 15(S)-HETE and the significant inhibition of LTB4 synthesis in IL-4-treated monocyte after challenge with opsonized zymosan. IL-4 treatment induced 15-LO enzymatic activity as well as 15-LO mRNA, but did not affect either 5-LO or FLAP mRNA expression in monocytes. Supernatant from IL-4-treated monocytes showed significantly lower neutrophil chemotactic activity than controls. 15(S)-HETE significantly inhibited LTB4 production induced by A23187-stimulated human monocytes without affecting AA release. IL-4-induced expression of 15-LO in monocytes caused a significant reduction of LTB4 production. Whereas this effect did not reflect changes in 5-LO and FLAP mRNA expression, synthetic 15(S)-HETE was able to significantly inhibit the synthesis of LTB4, without affecting AA release. (literal)
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