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Role of survivin in cytokinesis revealed by a separation-of-function allele (Articolo in rivista)

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Role of survivin in cytokinesis revealed by a separation-of-function allele (Articolo in rivista) (literal)

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Szafer-Glusman E, Fuller MT, Giansanti MG. (2011)
Role of survivin in cytokinesis revealed by a separation-of-function allele
in Molecular biology of the cell; Editore: American Society for Cell Biology, - Bethesda, MD, Bethesda, MD (Stati Uniti d'America)
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Departments of Developmental Biology and Genetics, Stanford University School of Medicine, Stanford, CA 94305; Istituto di Biologia e Patologia Molecolari del Consiglio Nazionale delle Ricerche, Dipartimento di Biologia e Biotecnologie C. Darwin, Università di Roma \"Sapienza,\" 00185 Rome, Italy (literal)

Titolo

Role of survivin in cytokinesis revealed by a separation-of-function allele (literal)

Abstract

The chromosomal passenger complex (CPC), containing Aurora B kinase, Inner Centromere Protein, Survivin, and Borealin, regulates chromosome condensation and interaction between kinetochores and microtubules at metaphase, then relocalizes to midzone microtubules at anaphase and regulates central spindle organization and cytokinesis. However, the precise role(s) played by the CPC in anaphase have been obscured by its prior functions in metaphase. Here we identify a missense allele of Drosophila Survivin that allows CPC localization and function during metaphase but not cytokinesis. Analysis of mutant cells showed that Survivin is essential to target the CPC and the mitotic kinesin-like protein 1 orthologue Pavarotti (Pav) to the central spindle and equatorial cell cortex during anaphase in both larval neuroblasts and spermatocytes. Survivin also enabled localization of Polo kinase and Rho at the equatorial cortex in spermatocytes, critical for contractile ring assembly. In neuroblasts, in contrast, Survivin function was not required for localization of Rho, Polo, or Myosin II to a broad equatorial cortical band but was required for Myosin II to transition to a compact, fully constricted ring. Analysis of this \"separation-of-function\" allele demonstrates the direct role of Survivin and the CPC in cytokinesis and highlights striking differences in regulation of cytokinesis in different cell systems. (literal)

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