Horizontal gene transfer from genetically modified tomato plants to Pseudomonas syringae pv. tomato and Ralstonia solanacearum. (Abstract in rivista)

Type
Label
  • Horizontal gene transfer from genetically modified tomato plants to Pseudomonas syringae pv. tomato and Ralstonia solanacearum. (Abstract in rivista) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Alternative label
  • COZZOLINO L., ZOINA A., RAIO A. (2007)
    Horizontal gene transfer from genetically modified tomato plants to Pseudomonas syringae pv. tomato and Ralstonia solanacearum.
    in SIPaV XIV National Meeting,, Perugia, 2007
    (literal)
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  • COZZOLINO L., ZOINA A., RAIO A. (literal)
Convegno
  • SIPaV XIV National Meeting, (literal)
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  • Perugia (literal)
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  • Journal of Plant Pathology , 89 (3, Supplement), S36. SIPaV XIV National Meeting, Perugia, 19-21 September 2007. (literal)
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  • Since antibiotic resistance markers are most frequently used for plant transformation, there is considerable concern about the possibility that these genes are transferred from transgenic plants to bacteria. In fact, bacteria that have developed a state of competence, may integrate foreign DNA into their genome by natural transformation and then express the newly acquired genes. To appraise the possibility that recombinant DNA will be transferred from genetically modified (GM) tomato plants to Pseudomonas syringae pv. tomato and Ralstonia solanacearum, both bacteria were first made competent to recombination, then transformed by inserting two different broad host range plasmids containing a deleted nptII genes into the cells. For these experiments transgenic tomato plants (cv. BetterBoy) transformed with prosystemin c-DNA and with nptII as gene marker were used. Restoration of 317 bp deleted nptII gene in Pseudomonas syringae pv. tomato (pBBRiTp DnptII) was detected, with extremely low frequency, after experiments of filter transformation with DNA from GM tomato plants conferring Kanamycin resistance. No kanamycin-resistant transformants were detected for Ralstonia solanacearum (pLaf DnptII). Probably this species did not develop DNA uptake competence under our experimental conditions. These preliminary results suggest that horizontal gene transfer may occur if homologous sequences are present both in the plant genome and in the phytopathogenic bacterium DNA. (literal)
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  • - Zoina A., ARBOPAVE, Università di Napoli “Federico II”, Portici (NA), Italy -Raio A., CNR, Istituto per la Protezione delle Piante, Sez. di Firenze, Italy (literal)
Titolo
  • Horizontal gene transfer from genetically modified tomato plants to Pseudomonas syringae pv. tomato and Ralstonia solanacearum. (literal)
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