Characterization of a quorum sensing controlled promoter: the phenazine operon promoter in Pseudomonas aureofaciens 30-84. (Abstract/Poster in atti di convegno)

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  • Characterization of a quorum sensing controlled promoter: the phenazine operon promoter in Pseudomonas aureofaciens 30-84. (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Alternative label
  • Puopolo G., Raio A., Zoina A., Pierson III L.S. (2006)
    Characterization of a quorum sensing controlled promoter: the phenazine operon promoter in Pseudomonas aureofaciens 30-84.
    in 13th Congresso Nazionale SIPaV, Foggia
    (literal)
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  • Puopolo G., Raio A., Zoina A., Pierson III L.S. (literal)
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  • Journal of Plant Pathology, 88 (3, Supplement), S55. 13th Congresso Nazionale SIPaV, 12-15 settembre 2006, Foggia, Italy. (literal)
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  • Phenazines are broad host range antibiotics produced by several bacterial species, including Pseudomonas and Streptomyces. These compounds play a key role in the antagonistic activity of the biocontrol strain P. aureofaciens 30-84 towards Gaeumannomyces graminis var. tritici, the causal agent of wheat take all disease. Strain 30-84 contains a seven-gene operon (phzXYFABCD) responsible for the biosynthesis of three phenazines. Expression of the phenazine operon is dependent on the PhzR/PhzI quorum sensing located upstream. The gene phzI encodes an AHL synthase responsible for the production of the signal molecule hexanoyl homoserine lactone (HHL). Once HHL reaches a threshold density it is believed to bind and activate PhzR, the transcriptional factor encoded by phzR, resulting in PhzR binding to the phenazine operon promoter and consecutive phenazine production. However, little is known regarding the functional phenazine promoter in strain 30-84. To further investigate the phenazine promoter, the region between phzR and phzX was characterized. Subclones of the phenazine promoter were cloned upstream of promotorless reporter genes in order to identify the smallest and most active region sufficient for phenazine transcription. Results showed that a 20 bp inverted repeat region (the phz box) proximal to the potential -35 position of the phenazine promoter is required for transcription. This work represents a first insight in the characterization of the quorum sensing controlled promoter of the phenazine operon in strain 30-84. Understanding phenazine gene regulation may be useful for the improvement of biological control in organic agriculture. (literal)
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  • - Puopolo G., Zoina A., ARBOPAVE, Università di Napoli “Federico II”, Portici (NA), Italy -Raio A., CNR, Ist. Protezione Piante - Sez. di Firenze, Italy -Pierson L.S., Division of Plant Pathology and Microbiology - University of Arizona, Tucson AZ, USA (literal)
Titolo
  • Characterization of a quorum sensing controlled promoter: the phenazine operon promoter in Pseudomonas aureofaciens 30-84. (literal)
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